中国人早发及多发糖尿病家系HNF-1α基因突变的筛查

目的　了解中国人早发及多发糖尿病家系中肝细胞核因子HNF 1α基因突变发生情况。方法　应用聚合酶链反应 单链构象多态性技术和测序方法对 2 47名无亲缘关系上海地区中国人 [其中 93名为正常对照者 ,15 4例为早发和 (或 )多发糖尿病家系先证者 ]进行HNF 1α基因启动子区、10个外显子及其侧翼内含子区筛查。结果　在 15 4例糖尿病家系先证者者中见到 14种碱基改变。 3种改变未见于 93名非糖尿病者 ,其中启动子区nt -12 8T→G和IVS2nt 2 1G→A未见报道 ,且在家系中表现为与糖尿病共分离 ;另 11种碱基改变 ,它们的基因型和等位基因频率在糖尿病者及非糖尿病者间差异未见显著性 ,且各变异与血糖、胰岛素、C 肽及空腹血脂谱等临床变量均无相关。结论　HNF 1α基因突变不是上海地区中国人早发及多发糖尿病的主要原因。

Scanning HNF-1α gene mutation in Chinese early-onset and/or multiplex diabetes pedigrees

OBJECTIVE: To investigate the prevalence of mutations of hepatocyte nuclear factor (HNF)-1 alpha gene in Chinese families with early-onset and/or multiplex diabetes mellitus. METHODS: The studied population consisted of 247 unrelated Chinese residents in Shanghai, including 93 healthy controls and 154 probands of early-onset and/or multiplex diabetes pedigrees. The ten exons, flanking introns and minimal promoter region of HNF-1 alpha gene were screened using polymerase chain reaction-single strand conformation polymorphism and DNA sequencing. RESULTS: Fourteen substitutions were identified in 154 probands. Three variants were not observed in 93 healthy controls. Two of them (nt-128T-->G IVS2 nt+21G-->A) were not reported previously and all co-segregated with diabetes. The genotype and allele frequencies of the other eleven variants in the diabetic patients were not significantly different from those in the healthy controls. There were no significant relationships between the eleven variants of HNF-1 alpha gene and clinical variables (plasma glucose, insulin, C-peptide and fasting lipid profile). CONCLUSION: HNF-1 alpha gene is not a major cause of early-onset or multiplex diabetes pedigrees in this Chinese population in Shanghai.