己酮可可碱对大鼠内毒素性急性肺损伤iNOS和NO的影响

目的 :探讨己酮可可碱 (PTX)对大鼠内毒素性急性肺损伤 (ALI)iNOS和NO的影响。方法 :采用大鼠内毒素ALI模型。 2 4只SD大鼠随机分为生理盐水对照组 (CON)、内毒素组 (LPS)和PTX组 ,每组 8只。观察PTX对氧合指数 (PaO2 /FiO2 )、支气管肺泡灌洗液 (BAL)中蛋白含量、肺组织iNOS、NO3- /NO2 - 、髓过氧化物酶 (MPO)活性的影响 ,计算肺湿 /干比值(W /D)并行肺组织病理学检查。结果 :PTX组自 2h起各时间点PaO2 /FiO2 均高于LPS组 (P <0 0 5 ) ,W /D、BAL中蛋白含量、肺组织iNOS、NO含量和MPO活性均较LPS组显著降低 (P <0 0 5 )。病理学检查显示PTX组肺组织损伤程度较LPS组减轻。结论 :PTX对内毒素性ALI的保护作用可能与其抑制肺组织iNOS活性和减少NO生成有关。

THE EFFECTS OF PENTOXIFYLLINE ON NITRIDE OXIDE SYNTHASE AND NITRIDE OXIDE IN RATS WITH ENDOTOXIN INDUCED ACUTE LUNG INJURY

Objective:To Investigate the effects of pentoxifylline (PTX) on inducible nitride oxide synthase and nitride oxide in rats with endotoxin induced acute lung injury.Methods:24 Sprague-Dawley rats were divided randomly into 3 groups:LPS group,pentoxifylline group and control group.Models of endotoxin induced ALI were used to observe the effect of PTX on PaO 2/FiO 2,changes of albumin contents in bronchoalveolar lavage fluid (BAL).Lung inducible nitric oxide synthnase(iNOS)activity and nitric oxide level and myeloperoxidase(MPO) activity were analyzed.The lung wet/dry weigh were also calculated.The histological change of lung were observed under light microscope.Results:The PaO 2/FiO 2 of PTX group was significant higher than that in LPS group from 2h(P<0.05).In contrast with group LPS, the W/D,albumin contents in BAL,iNOS and MPO activity,NO level of group PTX were reduced significantly(P<0.01).The histological changes of lung in group PTX is milder than that in group LPS.Conclusion:The protection of PTX on endotoxin induced lung injury is related to the inhibition on iNOS activity and NO lever in rat lungs.