Vogt-Koyanagi-Harada综合征与HLA-DRB基因的相关性

从基因水平研究汉族ＶＫＨ综合征（Ｖｏｇｔ－Ｋｏｙａｎａｇｉ－Ｈａｒａｄａｓｙｎｄｒｏｍｅ）患者发病的免疫遗传基因。方法应用聚合酶链反应（ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ,ＰＣＲ）检测５１例汉族ＶＫＨ患者及６２例正常人的ＨＬＡ－ＤＲＢ基因,并进一步应用序列特异的寡核苷酸探针（ｓｅｑｕｅｎｃｅｓｐｅｃｉｆｉｃｏｌｉｇｏｎｕｃｌｅｏｔｉｄｅｐｒｏｂｅ,ＳＳＯＤ）斑点杂交法及限制制性片段长度

Association of HLA-DRB genes with Vogt-Koyanagi-Harada syndrome in a Chinese Han population

Objective To investigate the molecular genetic background of VKH syndrome in Han Chinese by HLA DRB genotyping. Methods HLA DRB genotyping was performed by PCR SSOP method, then DR4 alleles were analyzed by SSOP and RFLP methods.Results The frequency of DRB1*0405 was significantly higher in VKH patients than that in normal controls (80 4% vs 8 1%, P c<10 -7 ,RR=46 7),so was the frequency of DRB1*15 in DRB1*0405 negative individuals ( P c<0 05,RR=6 0).On the other hand, the frequencies of DRB1*14 and DRB1*08 were significantly lower in VKH patients than that in normal controls. Comparing the clinical features between DRB1*0405 positive and negative VKH patients, no significant differences were found. Conclusions (1) DRB1*0405 and DRB1*15 are closely associated with the susceptibility to VKH syndrome,DRB1*0405 may be the major susceptible gene and DRB1*15 may be the minor;(2) DRB1*14 and DRB1*08 are negatively associated with the susceptibility to VKH syndrome, suggesting that they may be the resistant genes;(3) DRB1*0405 is not related to the clinical features, incidence of ocular complications as well as visual prognosis.