| 腺病毒载体介导的单纯疱疹病毒-胸苷激酶基因杀伤人膀胱癌细胞的实验研究 |
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| 引用本文： | 潘浩,温端改,郭震华,严春寅,浦金贤,侯建全. 腺病毒载体介导的单纯疱疹病毒-胸苷激酶基因杀伤人膀胱癌细胞的实验研究[J]. 中国血液流变学杂志, 2007, 17(1): 14-15,48 |
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| 作者姓名： | 潘浩 温端改 郭震华 严春寅 浦金贤 侯建全 |
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| 作者单位： | 苏州大学附属第一医院泌尿外科,江苏苏州,215006 |
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| 摘 要： | 目的探讨HSV-tk/GCV系统基因治疗膀胱癌细胞的方法及结果。方法与结果采用了带有CMV启动子驱动HSV-tk基因的重组腺病毒载体,体外转染HTB9细胞后对GCV敏感性增强。当MOI=100时,HTB9细胞的IC50为2.5mg/L,病毒浓度降低,GCV对细胞的杀伤效率亦降低。将不同比例的tk 细胞和tk-细胞混合后加入10mg/L GCV,发现HSV-tk/GCV系统有很强的“旁观者效应”。结论HSV-tk/GCV系统是一种有效、安全地治疗膀胱癌的新方法。
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| 关 键 词： | 膀胱癌 基因治疗 腺病毒 单纯疱疹病毒-胸苷激酶基因 旁观者效应 |
| 文章编号： | 1009-881X（2007）01-0014-02 |
| 修稿时间： | 2006-09-15 |
Study of Adenovirus-Mediated HSV-tk Gene Therapy for Bladder Cancer Cell Line |
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| PAN Hao, WEN Duan-gai, GUO Zhen-hua, et al. Study of Adenovirus-Mediated HSV-tk Gene Therapy for Bladder Cancer Cell Line[J]. Chinese Journal of Hemorheology, 2007, 17(1): 14-15,48 |
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| Authors: | PAN Hao WEN Duan-gai GUO Zhen-hua et al |
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| Affiliation: | Department of Urology, the First Hospital Affiliated to Suzhou University, Suzhou 215006 |
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| Abstract: | Objective To study the new way of adenovirus-mediated gene therapy for bladder cancer.Methods and Results We used the replication-defective adenovirus carring the HSV-tk gene(Ad.tk) under control of the cytomegalovirus(CMV).In vitro experiments,the HTB9 cells transferred by tk gene were killed in the presence of GCV treatment.When tk cells were mixed with their parental cells at various ratios,GCV not only killed tk cells,but neighboring cells without tk gene also killed by bystander effect.Conclusion Adenovirus-mediated HSV-tk/ GCV system may be used as apromising suicide gene therapy for bladder carcinoma. |
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| Keywords: | Bladder carcinoma Herpes simplex thymidine kinase Gene therapy Adenovirus Bystander effect |
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