| 自杀基因HSV-TK对肺癌细胞增殖活性影响的实验研究 |
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| 引用本文： | 于雁,孙凤林,陈雪松. 自杀基因HSV-TK对肺癌细胞增殖活性影响的实验研究[J]. 实用肿瘤学杂志, 2005, 19(1): 11-13 |
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| 作者姓名： | 于雁 孙凤林 陈雪松 |
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| 作者单位： | 哈尔滨医科大学附属肿瘤医院,哈尔滨,150040 |
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| 基金项目： | 黑龙江省卫生厅科研基金资助,编号 2 0 0 0 -0 10 |
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| 摘 要： | 目的探讨自杀基因HSV-TK对肺癌细胞增殖活性影响.方法利用嗜银染色AgNOR观察肺癌细胞系Anip973在经阳离子脂质体介导的自杀基因系统HSV-TK转染后应用阿昔洛韦(ACV),观察细胞形态并计数,观察其增殖活性的变化.结果经AgNOR嗜银染色72h测定,其结果表明,G、G-Lipo、G-P细胞AgNOR平均数无明显差别,而G-pTK细胞的AgNOR平均数明显低于前三种细胞,具有显著差异.结论自杀基因HSV-TK/GCV系统对肺癌细胞株Anip973的细胞增殖活性具有抑制作用,对其临床应用具有理论意义.
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| 关 键 词： | 自杀基因 肺癌 AgNOR 增殖活性 |
| 修稿时间： | 2004-09-15 |
The proliferative activity influence of HSV-TK/ACV transfected by cationic liposme to lung caner cell |
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| YU Yan,SUN Fenglin,Chen Xuesong. The proliferative activity influence of HSV-TK/ACV transfected by cationic liposme to lung caner cell[J]. Journal of Practical Oncology, 2005, 19(1): 11-13 |
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| Authors: | YU Yan SUN Fenglin Chen Xuesong |
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| Affiliation: | YU Yan,SUN Fenglin,CHEN XuesongThe Affiliated Tumor hospital of Harbin Medical University,Harbin 15 0040 |
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| Abstract: | Objective To evaluate the th e proliferative activity influence of HSV-TK/ACV transfected by cationic liposom e to lung cancer cell. Methods This study was carried out by silver staining to lung cell line Anip 973 whcih was tranfected HSV-TK gene and treated with ACV, then count AgNOR. Results After 72 h silver staining, AgNOR were counted, the average number of G?G-Lipo?G-p wer e no significan difference and the average AgNOR number of the cell which was tr ansfected TK gene was significantly low to the others. Conclusion The suicide gene HSV-TK can inhibit the proliferative activity of lung cancer cell line Anip 973, and it is promising to be used in clinical genetherap y. |
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| Keywords: | Suicide gene Lung carcinoma AgNOR Proliferative activity |
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