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白细胞介素10对小鼠单纯疱疹性角膜基质炎作用的实验研究
作者姓名:Xia LK  Gao DW  Pu W  Zhang JS
作者单位:1. 110004,沈阳,中国医科大学附属第二医院眼科
2. 中国医科大学附属第一医院眼科
摘    要:研究白细胞介素10(IL-10)在小鼠单纯疱疹性角膜基质炎(herpetic stromal keratitis,HSK)中的作用。方法雌性BALB/c小鼠80只分为2组,每组40只;将单纯疱疹病毒1型接种于小鼠角膜上建立HSK动物模型;分别于鼠角膜接种病毒前的6h、当日及接种后2、4d,向实验组鼠的角膜内注射重组IL-10 20ng,腹腔内注射鼠重组IL-10 500 ng;对照组同步注射生理盐水;观察IL-10对小鼠HSK的发病率、临床特征、角膜病毒滴度、角膜细胞因子含量、角膜病理改变及迟发型超敏反应(DTH)的影响。结果IL-10降低了HSK发病率,减轻了HSK角膜混浊程度、角膜新生血管化程度及角膜内炎性细胞浸润,降低了角膜内IL-2和IL-6的含量,抑制了鼠的DTH反应。IL-10不影响病毒在角膜内的复制和清除。结论IL-10可抑制鼠的DTH反应和HSK的发病进展。

关 键 词:白细胞介素10  小鼠  单纯疱疹性角膜基质炎  HSK  细胞因子类
修稿时间:2003年5月7日

Suppression effects of interleukin-10 on mouse herpetic stromal keratitis
Xia LK,Gao DW,Pu W,Zhang JS.Suppression effects of interleukin-10 on mouse herpetic stromal keratitis[J].Chinese Journal of Ophthalmology,2003,39(10):592-596.
Authors:Xia Li-kun  Gao Dian-wen  Pu Wei  Zhang Jin-song
Institution:Department of Ophthalmology, Second Affiliated Hospital, China Medical University, Shenyang 110004, China. xialk@online.ln.cn
Abstract:Objective To investigate the role of interleukin 10 (IL 10) in herpetic stromal keratitis (HSK). Methods Eighty BALB/c mice were divided into two groups and infected with HSV 1 Mckrae strain by corneal scarification. Murine rIL 10 (20 ng/ml) was inoculated intracorneally 6 h before and again on days 0, 2 and 4 after topical HSV 1 corneal infection. rIL 10 (500 ng) was given intraperitoneally in treated mice simultaneously. The same amount of saline was injected into the control mice. The effects of IL 10 on HSK were evaluated. Results In the IL 10 treated animals, the onset of HSK was delayed, corneal stromal opacification and neovascularization were reduced, extensive cellular infiltrates in the cornea were prevented and delayed type hypersensitivity (DTH) was weakened. Examination of pro inflammatory cytokine levels in the cornea 10 days after the infection revealed that the amounts of IL 2 and IL 6 were lower than those found in the controls. IL 10 did not suppress the viral replication nor did it eliminate the virus from IL 10 treated eyes, as compared with the controls. Conclusions IL 10 treatment can suppress DTH responsiveness and the production of certain cytokines by corneal cells. It delays the onset and decreases the severity of HSK.
Keywords:Keratitis  herpetic  Interleukin  10  Cytokines
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