抗骨痛注射液对大鼠踝关节炎性变化及肿胀程度的影响

背景:应用抗骨痛注射液治疗关节肿痛经过对中药的多次筛选及10余年的临床观察,在消除关节肿痛、颈椎病、骨关节病症状及体征方面效果较为突出。为进一步研究抗骨痛对关节肿痛、颈椎病、骨关节病治疗作用的机制,作者做了动物实验观察。目的:探讨中药抗骨痛注射液治疗局部软组织增生、水肿、刺痛或压迫神经的作用机制。设计:随机对照研究。单位:解放军第二○二医院理疗科,上海中医药大学实验动物部。材料:实验于2002-05/2003-01在上海中医药大学实验动物部完成,并由该部提供4月龄健康雌性SD大鼠21只,清洁级,按体质量随机抽样分成3组,即对照组、地塞米松组、抗骨痛组,每组7只大鼠。方法:对照组,腹腔注射生理盐水6mL;地塞米松组,腹腔注射0.08g/L地塞米松6mL;抗骨痛组,腹腔注射抗骨痛注射液6mL,0.5h后,在3组大鼠右踝关节周围每只注入0.3mL的尿酸钠(25g/L)造成关节炎,分别在实验前和实验后10,20h检测右踝关节的周径。主要观察指标:实验前和实验后10,20h大鼠右踝关节的周径。结果:3组大鼠右踝关节在尿酸钠的作用下发生了无菌性关节炎,局部肿胀。由于各组在腹腔内注射了不同药物,而使关节肿胀不同。实验后10h关节肿胀程度比较:对照组、地塞米松组及抗骨痛组的关节周径分别为(57.80±2.26),(31.12±5.11)及(43.86

Effect of kanggutong injection on arthritic change of ankle and severity of swelling in rats

BACKGROUND: By several screenings and more than ten-year clinical observation, it is discovered that the outstanding results have been achieved by the treatment of joint swelling and pain with Kanggutong injection (KGT)in the aspects of releasing joint swelling and pain, cervical spondylosis, the symptoms and physical signs of osteoarthropathy. In order to study on the mechanism of KGT on joint swelling and pain, cervical spandylosis and osteoarthropathy, the writers carried on the animal experiment.OBJECTIVE: To probe into the mechanism of KGT injection on the treatment of local soft tissue hyperplasia, edema, pricking pain or neural compression.DESIGN: A randomized controlled study.SETTING: Department of Physiotherapy, the 202 Hospital of Chinese PLA,Department of Experimental Animal, Shanghai University of Traditional Chinese Medicine.PARTICIPANTS: The experiment was accomplished in the Department of Experimental Animal of Shanghai University of Traditional Chinese Medicine from May 2002 to January 2003. The department provided 21 normal female SD rats, aged 4 months, clean grade, which were divided into 3 groups at random, named as control group, dexamethasone(DXM) group and KGT group, 7 rats in each group.INTERVENTIONS: Abdominal injection with physiological saline 6ml was done in control, with DXM 0.08 g/L, 6 mL in DXM group and with KGT 6 mL in KGT group. 0.5 hour later, uric acid sodium 0. 3 mL (25 g/L) was injected peripherally on the right ankle joint of each rat in 3 groups to induce arthritis. The perimeter of the right ankle joint was tested before and 10 and 20 hours after experiment.MAIN OUTCOME MEASURES: The perimeter of the right ankle joint before and 10 and 20 hours after experimentRESULTS: The right ankle joints of rats in the 3 groups were induced aseptic arthritic and local swelling by uric acid sodium. But the severity of swelling was various due to intra-abdominal injection of different medicines. Comparison of joint swelling severity 10 hours after experiment: the joint perimeters in the control, DXM and KGT groups were(57.80 ±2.26), (31.12 ±5.11) and (43.86 ±4. 92) mm respectively. Compared with the control group, the differences in DXM and KGT groups were significant( t = 12.28, P ＜ 0.01; t= 7.56, P ＜ 0. 01 ). Comparison of joint swelling severities 20 hours after experiment: the joint perimeters in the control, DXM and KGT groups were (49.86 ± 6.28), (6.82 ± 4.92) and(26.71 ± 5.42) mm respectively. Compared with the control group, the differences in DXM and KGT groups weresignificant(t= 14. 27, P ＜0.01;t =7.38, P ＜0.01).CONCLUSION: The same as DXM, KGT injection acts on anti-inflammation and anti-swelling for arthritic changes and swelling.