针刺对高脂血症小鼠血脂、抗氧化能力及血管内皮保护功能的影响（英文）

目的:观察针刺对高脂血症小鼠的疗效及作用机制。方法:将雄性清洁级昆明小鼠120只,随机分为正常组、模型组各40只,针刺组和药物组各20只。除正常组外采用高脂饮食饲料喂养制备高脂血症小鼠模型。造模第15天检测正常及造模动物各20只的血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C);同时针刺组开始电针双侧"丰隆""曲池""三阴交"穴10 min,每日1次,连续干预10 d;药物组灌服辛伐他汀,每日1次,连续10 d。治疗结束后检测各组血清TC、TG、LDL-C、HDL-C及丙二醛(MDA)、一氧化氮(NO)、内皮素(ET)含量和超氧化物歧化酶(SOD)活力。结果:第15天,模型组小鼠血清TG、TC、LDL-C含量均高于正常组(P<0.05),HDL-C含量低于正常组(P<0.01)。治疗10 d后,针刺组、药物组小鼠血清TG、TC、LDL-C以及MDA、ET含量均低于模型组,血清HDL-C、NO含量以及SOD活力高于模型组(P<0.05,P<0.01),且针刺组的改善作用优于药物组(P<0.05,P<0.01)。结论:针刺治疗能够调节高脂血症小鼠血脂代谢,抵抗脂质过氧化,并具有保护血管内皮功能的作用。

Effects of acupuncture on blood-lipids, anti-oxidizing ability and vascular endothelial protective function in hyperlipemia mice

Objective To observe therapeutic effects of acupuncture on the mouse of hyperlipemia and to explore the mechanisms. Methods One hundred and twenty Kunming mice, male, sanitary degree, were randomly divided into normal group (n=40), a model group (n=40), an acupuncture group (n=20) and a medicine group (n=20). Except the normal group, the mice were fed with high fat forage to prepare mouse hyperlipemia model. On the 15th day of modeling, serum total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C), high density lipoprotein-cholesterol (HDL-C) levels were detected in 20 normal mice and 20 model mice; electroacupuncture was given at bilateral "Fēnglóng" (丰隆 ST 40), "Qūchí" (曲池 LI 11), "Sānyīnjiāo" (三阴交 SP 6) in the acupuncture group, once a day, for 10 consecutive days; in the medicine group, the mice were intragastrically administrated with Simvastatin, once daily , for 10 consecutive days. After the end of treatment, serum TC, TG, LDL-C, HDL-C, malondialdehyde (MDA), nitric oxide (NO) and endothelin (ET) contents and superoxide dismutase (SOD) activities were detected in the groups. Results On the 15th day of modeling, in the model group serum TG, TC, LDL-C contents were significantly higher (all P<0.05) and HDL-C content was significantly lower (P<0.01) than those in the normal group. After treatment for 10 days, in the acupuncture group and the medicine group serum TG, TC, LDL-C, MDA and ET contents were significantly lower and serum HDL-C and NO contents and SOD activities were significantly higher than those in the model group (P<0.05, P<0.01), and the improving action in the acupuncture group was better than that in the medicine group (P<0.05, P<0.01). Conclusion Acupuncture can regulate fat metabolism, resist lipid peroxidation and protect vascular endothelial function in the mouse of hyperlipemia.