人肝刺激因子的提取及其保肝作用的初步观察

采用水囊引产眙儿(胎龄4～6个月),提取肝刺激因子(hHSS),用~3H—胸腺嘧啶核苷测其活性,观察到 hHSS 可使34%肝切除的大鼠对~3H—胸腺嘧啶核苷掺入再生肝 DNA 率明显增加。将 hHSS 预先注入半乳糖胺损伤的小鼠体内,结果观察到:hHSS 极明显地降低半乳糖胺中毒的死亡率(P<0.01),明显地抑制半乳糖胺所致的血清 GPT 和 GOT 的高水平。电镜检查表明,hHSS 可保护肝细胞的超微结构(线拉体、内质网等)免受半乳糖胺的损伤。这些结果证明,从人胎肝中可提取 hHSS,而 hHSS 具有保护肝脏免受肝毒剂—半乳糖胺的毒害作用。

Isolation of human hepatic stimulator substance and the preliminary study of its protective action(Abstract)

The human hepatic stimulator substance(hHSS)had been isolated and partial purified from human fetal liver(4-6 month age),and biological activity of hHSS was demonstrated by means of~3H-thymidine incorpo- ration into hepatocyte DNA when hHSS injected intraperitoneally into 34% hepatectomy rats.We further observed that hHSS could decrease the mortality induced by D-galactosamine poisoning mice,and suppress the high level of sGPT and sGOT evoked by D-galactosamine intoxica- tion.The electromicroscope examination show that hHSS lessen the structural damage of liver induced by D-galactosamine.The above- mentioned results suggest that hHSS may be isolated from human fetal liver,and it has dramatic protective effect on liver against D-galacto- samine injure.