不同持续时间的周期性压力对构建组织工程软骨的影响 |
| |
引用本文: | 盛英俊,范卫民,马益民,高峰,高共鸣. 不同持续时间的周期性压力对构建组织工程软骨的影响[J]. 中华创伤骨科杂志, 2006, 11(1): 546-550. DOI: 10.3760/cma.j.issn.1671-7600.2009.06.012 |
| |
作者姓名: | 盛英俊 范卫民 马益民 高峰 高共鸣 |
| |
作者单位: | 南京医科大学第一附属医院骨科,210029; |
| |
基金项目: | 国家自然科学基金江苏省自然科学基金 |
| |
摘 要: | Objective To determine the best duration for exerting the cyclic pressure under which the tissue-engineered cartilage is constructed. Methods Free chondrocytes isolated from rabbit articular cartilage were seeded into polylactic acid-co-glycolic acid(PLGA) scaffolds after expansion in vitro, and ran-domized into 4 groups. In Groups 1 to 3, chondrocytes were cultured under daily cyclic pressure (0 ~ 200 kPa, 0.1Hz) for 4 hours, 8 hours, 12 hours respectively; Group 4 was a control in which no pressure was exerted. In each group, after 2 weeks of culture, the tissue engineered cartilages were observed in vitro and assessed by his-tological staining of liE. Next, the content of DNA and the secretion of type Ⅱ collagen and GAG in cartilages were detected quantitatively. Results Under the daily cyclic pressure (0 ~200 kPa, 0.1 Hz), the scaf-fold-chondrocytes complex in the group of 8 hours got the largest volume, smooth, lucidus, and elastic surface, the most queuing chondrocytes, and the highest content of type Ⅱ collagen and GAG (P < 0.01). Conclusions Since chondrecytes are baro-senstive, the metabolism of chondrocytes can be affected by the time of cyclic pressure. Under the effect of 0 ~200 kPa, 0.1Hz, the daily cyclic pressure of 8 hours may be optimal for chondrocytes to multiply and synthesize extracellular matrixes such as type Ⅱ collagen and GAG.
|
关 键 词: | 软骨细胞 压力 频率 时间 组织工程 |
Tissue engineered cartilage in response to different durations of the cyclic pressure |
| |
Abstract: | |
| |
Keywords: | ChondrocytesPressureFrequencyTimeTissue engineering |
|
|