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| 蚯蚓纤溶酶化学修饰的研究 | |
| 引用本文： | 莫宏波,伍晓斌,徐凤彩.蚯蚓纤溶酶化学修饰的研究[J].药物生物技术,2004,11(5):306-310. |
| 作者姓名： | 莫宏波 伍晓斌 徐凤彩 |
| 作者单位： | 1. 暨南大学医学院,广东,广州,510632 2. 中国科学院微生物研究所,北京,100080 3. 华南农业大学生命科学院,广东,广州,510642 |
| 基金项目： | 广东省高教厅基金(200001) |
| 摘 要： | 以三聚氰氯为活化剂，采用活化聚乙二醇(PEG)对环毛蚯蚓纤溶酶进行化学修饰，优化了修饰条件，得到了PEG-EFE的加合物(修饰酶)，并研究了PEG-EFE的部分酶学性质。修饰酶的活力为天然酶的85％，结果表明酶的活性中心基本保持不变。对修饰酶的残留氨基测定表明，70％的可滴定氨基参加了反应，且EFE的活性改变较小，可以推测PEG是连接在蛋白质表面上的。 |
| 关 键 词： | 修饰酶 改变 PEG 化学修饰 天然 活性中心 氨基 蚯蚓纤溶酶 酶学性质 蛋白质 |
| 文章编号： | 1005-8915(2004)05-0306-05 |
Studies on Chemical Modification of Fibrinolytic Enzyme from Pheretima |
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| MO Hong bo ,WU Xiao bin ,XU Feng cai.Studies on Chemical Modification of Fibrinolytic Enzyme from Pheretima[J].Pharmaceutical Biotechnology,2004,11(5):306-310. | |
| Authors: | MO Hong bo WU Xiao bin XU Feng cai |
| Institution: | MO Hong bo 1,WU Xiao bin 2,XU Feng cai 3 |
| Abstract: | EFE was separated from Pheretima and modified with activated PEG. The optimum conditions for chemical modification were selected. The activity of modified EFE is 85% of that of free EFE. It means that the activity site of EFE almost weren't changed. The modification rate of amino residues is 70%. We could guess that activated PEG connected with the surface of EFE protein. It showed the modified EFE could improve its thermal and pH stability. |
| Keywords: | Chemical modification Fibrinolytic enzyme Pheretima |
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