Regional distribution of calcium elevation during sensory transduction in spider mechanoreceptor neurons |
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| Institution: | 1. University of Victoria-Genome British Columbia Proteomics Centre, Vancouver Island Technology Park, #3101-4464 Markham St., Victoria, BC V8Z 7X8, Canada;2. Department of Biochemistry and Microbiology, University of Victoria, Petch Building Room 207, 3800 Finnerty Rd., Victoria, BC V8P 5C2, Canada;1. Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China;2. University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100039, China;1. Department of Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China;2. Institute for Viral Hepatitis of Chongqing Medical University, Chongqing, China;3. Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China;1. OncoProteomics Laboratory, Department of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands;2. Department of Functional Genomics and Clinical Genetics, Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, VU University Amsterdam, VU University Medical Center, The Netherlands;3. Department of Clinical Chemistry, Neurochemistry Laboratory and Biobank, VU University Medical Center, Amsterdam, the Netherlands;1. Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, Córdoba, Spain;2. Red Española de Investigación en Patología Infecciosa (REIPI), Instituto de Salud Carlos III, Madrid, Spain;3. Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, USA;4. Unidad de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Instituto de Biomedicina de Sevilla/Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Sevilla, Spain;5. Departamento de Biología Celular, Fisiología e Inmunología, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, Córdoba, Spain;6. Departamento de Botánica, Ecología y Fisiología Vegetal, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, Córdoba, Spain;7. Departamento de Química Analítica, Universidad Complutense de Madrid, Madrid, Spain;8. Sección de Enfermedades Infecciosas Pediátricas e Inmunopatología, Hospital Universitario Infantil Virgen del Rocío, Sevilla, Spain;9. Department of Medicine and Microbiology and Immunology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY, USA;1. University of Victoria — Genome British Columbia Proteomics Centre, University of Victoria, Victoria, BC V8Z7X8, Canada;2. Center for Proteomics and Metabolomics, Leiden University Medical Center, The Netherlands;3. Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland;4. Department of Biochemistry & Microbiology, University of Victoria, Victoria, BC V8P 5C2, Canada |
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| Abstract: | Spider mechanosensory VS-3 neurons receive peripheral efferent synaptic modulation, with regional variations in the types of efferent synapses and transmitter receptors. VS-3 somata possess a voltage-activated calcium current, but the levels and time courses of calcium changes in other regions are unknown. The roles of calcium in these neurons are not completely understood, but could include modulation of both mechanosensitivity and response dynamics. Here, we measured calcium concentration rises caused by single, mechanically induced action potentials in VS-3 sensory dendrites, somata and axons, using Oregon Green BAPTA-1 fluorescence. Calcium concentration rose by ～1 nM following each action potential. Time courses of calcium rise and fall were similar in the three regions but the rise in amplitude was about 50% higher in the sensory dendrite than in the soma. Antibody to the CaV3.1(α1g) isotype of T-type calcium channel labeled all three neuronal regions. Some CaV3.1 labeling colocalized with synapsin labeling, suggesting that calcium channels play some part in efferent modulation. We conclude that mechanically stimulated action potentials start near sensory dendrite tips and pass rapidly through the neurons to the axons, activating low voltage activated calcium channels in all three regions and causing calcium concentration to rise rapidly in each region. These results suggest important roles for calcium in several stages of mechanosensation. |
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