碱性成纤维细胞生长因子对组织工程化外周神经的影响

目的　研究碱性成纤维细胞生长因子 (bFGF)和肝素与乳兔许旺细胞、去细胞基膜管、构成的复合型组织工程化外周神经桥接体修复兔正中神经缺损的效果。　方法　新西兰兔 48只 ,建立左侧上臂正中神经 3 0mm缺损模型 ,随机分为 4组 ,分别用去细胞基膜管种植许旺细胞并复合bFGF及肝素 (Hep)的桥接体 (A组 )、去细胞基膜管种植许旺细胞的桥接体 (B组 )、去细胞基膜管复合bFGF及Hep桥接体 (C组 )、自体神经 (D组 )修复神经缺损 ,于术后 1、3个月分别进行大体观察 ,Masson三色染色光镜观察神经再生、神经内胶原纤维形成及血管形成 ,3个月检测各组桥接体运动神经传导速度 ,并行透射电镜检查 ,称量指浅屈肌肌肉湿重 ,观察神经功能恢复。　结果　去细胞基膜管种植许旺细胞并复合bFGF及Hep的桥接体组 (A组 )神经再生及功能指标 (再生有髓神经纤维密度、平均髓鞘厚度、有髓纤维直径、运动神经传导速度、肌肉湿重恢复率 )与自体神经移植 (D组 )比较 ,差异无显著性 (P >0 .0 5 )。　结论　bFGF及肝素与许旺细胞、去细胞神经基膜管构成的复合型组织工程神经桥接体修复神经缺损能提高神经再生质量。

Effect of basic fibroblast growth factor in tissue engineering peripheral nerve bridges

Objective To investigate the effect of constructed artificial nerves compound of bFGF and heparin in repairing rabbits the nerve defects. Method Forty eight adult New Zealand rabbits were random divided into four groups(group A,B,C and D)and the models of injured median nerve of 30mm gap were made. Median nerve defects were repaired in each group. In group A, the artificial nerves composed of acellular nerve basal lamina tube, neonatal Schwann cells, bFGF and heparin were used to repair the nerve defects. In group B, the bridge consist of basal lamina tube and neonatal Schwann cells were used and group C exogenous bFGF and heparin were trapped in basal lamina tube bridges group D which used autologous nerve grafts served as controls. l month and 3 month postoperatively, the regeneration of the nerve bridges of each group were observed including general observation, histological examination and morphologic analysis, electrophysiological test and muscle wet weight resume rate. Result The parameter in group A(including the density of regenerative myelinated fibers, dimeter of myelinated fibers, thickness of myelin sheath, the motor nerve conduction velocity, and muscle wet weight) were not statistically different with group D ( P >0.05). Conclusion The nerve bridge compounded of bFGF heparin, acellular nerve basal lamina tube and Schwann cells can facilitate nerve regeneration. [Key words] Peripheral nerve; Tissue engineer; Acellular nerve basal lamina tube; Basic fibroblast growth factor(bFGF); Schwann cells