大豆异黄酮对膳食诱导胰岛素抵抗大鼠脂联素基因表达的影响

目的探讨大豆异黄酮(SIF)改善高脂高糖膳食诱导大鼠胰岛素抵抗(IR)状态的作用及可能机制。方法选用高脂高糖饲料诱导的IR雄性SD大鼠,根据胰岛素抵抗指数(IRI)随机分为模型对照组和3个SIF剂量组(50、150及450mgkgbw)。各组给予相应受试物1月后,试纸法检测各组大鼠血糖、放免法检测血胰岛素、酶免法检测血脂联素含量,实时定量RTPCR法检测肾周脂肪组织脂联素基因的mRNA水平。结果与模型对照组比较,450mgkgbw组能明显降低大鼠体重及内脏脂肪含量、提高脂肪组织脂联素基因mRNA的表达、促进脂联素的分泌。150mgkgbw和450mgkgbw组能显著降低血胰岛素水平及IRI。3个SIF剂量组的血糖与模型对照组比较无明显差异。IRI与血清脂联素水平存在着明显的负相关。结论SIF具有减少大鼠脂肪沉积、促进脂联素的分泌、提高胰岛素敏感性的作用。

Effects of soy isoflavone on gene expression of adiponectin in insulin-resistance rats induced by high-fat and high-sugar diet

Objective To study the effects and mechanisms of soy isoflavone (SIF) on improving insulin resistance(IR) status in IR rats induced by high-fat and high-sugar diet.Methods IR rats induced by high-fat and high-sugar diet were assigned into four groups and gavaged with SIF at doses of 0 (model control group), 50, 150 and 450mg/kg bw for 4 weeks, respectively. Insulin, glucose, adiponectin in serum and adiponectin mRNA in perirened adipocyte were detected.Results Compared with model control group, body weight and fat pad weight were lower, and serum adiponectin and adiponectin mRNA expression were higher in the 450mg/kg bw group, insulin and insulin-resistance index were lower in the 150mg/kg bw group and 450mg/kg bw. There is no differences in plasma glucose levels among groups. There is an negative correlation between insulin-resistance index and serum adiponectin level (=24.802-2.856X,r=-0.881,n=47,P<0.01). Conclusion These results suggest that soy isoflavone may decrease adipose deposition, increase serum adiponectin level, and ameliorate insulin sensitivity.