不同剂量环磷酰胺诱导正常小鼠免疫抑制的对比研究

目的观察不同剂量环磷酰胺对正常小鼠免疫抑制的影响。方法采取不同的时间给予正常小鼠腹腔内注射小同剂量的环磷酰胺(CY),观察对小鼠免疫学指标的影响。结果正常小鼠腹腔注射20、80、100mg/kg的CY后,各剂量组均出现不同程度的免疫抑制。结论给予正常小鼠腹腔注射20、80、100mg/kg的CY,可建立免疫抑制模型,并且连续3d腹腔注射剂量为80mg/(kg·d)1CY的效果盛明显。

Comparison of immunosuppression induced by different doses of cyclophosphamide in normal mice

objective To investigate the effects of cyclophosphamide (CY) on the immunosuppression in normal mice. Methods Different doses of CY were given to normal mice at different time-points, and the effects of CY on immunological indices of mice were observed. Results After injection of CY at doses of 20, 80, 100 mg/kg, all mice showed various degrees of immunosuppression. The rate of lymphocyte transformation, the levels of NK cell activity, the number of total T cells, and the rates of Th/Ts were significantly descended in low-dose treated mice ( P < 0.01) and high-dose trea-ted mice ( P < 0.05). But there was no significant difference between high-dose treated mice (80 mg/kg for 5 d) and the untreated control mice ( P > 0.05). Conclusion Immunosuppressive model has been established by intraperitoneal injection of CY at the doses of 20, 80, and 100 mg/kg, and the dose of 80 mg/kg for 3 d was the best dose for establishing the model.