| 硫磺熏蒸前后菊花的HPLC指纹图谱比较研究 |
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| 引用本文： | 刘晓,蔡皓,马晓青,裴科,蔡宝昌. 硫磺熏蒸前后菊花的HPLC指纹图谱比较研究[J]. 中药材, 2012, 0(5): 705-708 |
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| 作者姓名： | 刘晓 蔡皓 马晓青 裴科 蔡宝昌 |
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| 作者单位： | 南京中医药大学药学院;南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心 |
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| 基金项目： | 国家自然科学基金资助项目(30940093);江苏省自然科学基金资助项目(BK2009495) |
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| 摘 要： | 目的:建立菊花药材指纹图谱检测方法,并对硫磺熏蒸前后菊花药材的指纹图谱进行分析比较。方法:采用高效液相色谱法,色谱柱为Waters C18(250 mm×4.6 mm,5μm);流动相为乙腈-0.5%磷酸水溶液,梯度洗脱;柱温35℃,检测波长268 nm。结果:测定未经硫磺熏蒸和经过硫磺熏蒸的菊花药材样品共11批,建立了菊花药材HPLC指纹图谱的共有模式,确立12个共有峰,并且采用聚类分析方法对11批样品进行分析比较。结论:该方法准确可靠,重复性好,可用于硫磺熏蒸前后菊花药材的质量评价与控制。
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| 关 键 词： | 菊花 指纹图谱 硫磺熏蒸 高效液相色谱 |
Study on HPLC Fingerprints of Chrysanthemi Flos before and after Sulfur-fumigation |
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| LIU Xiao,CAI Hao,MA Xiao-qing,PEI Ke,CAI Bao-chang. Study on HPLC Fingerprints of Chrysanthemi Flos before and after Sulfur-fumigation[J]. Journal of Chinese medicinal materials, 2012, 0(5): 705-708 |
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| Authors: | LIU Xiao CAI Hao MA Xiao-qing PEI Ke CAI Bao-chang |
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| Affiliation: | 1,2(1.College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210046,China;2.Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing,Nanjing University of Chinese Medicine,Nanjing 210029,China) |
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| Abstract: | Objective:To evaluate the quality of Chrysanthemi Flos before and after sulfur-fumigation by analysis of HPLC fingerprints.Methods:RP-HPLC method was applied to establish the chromatographic fingerprint.The separation was performed on a Waters C18(250 mm × 4.6 mm,5 μm)column with a gradient elution composed of acetonitrile-aqueous solution containing 0.5% phosphoric acid.The column temperature was 35 ℃,the detective wavelength was set at 268 nm.Results:Eleven batches of Chrysanthemi Flos samples composed of both sun-dried and sulfur-fumigated were analyzed and evaluated respectively.The method of clustering analysis was used,and the chromatographic fingerprint common pattern was finally established.Conclusion:The HPLC fingerprint method is reliable and suitable for quality evaluation of Chrysanthemi Flos before and after sulfur-fumigation. |
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| Keywords: | Chrysanthemi Flos Fingerprint Sulfur-fumigated RP-HPLC |
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