磺基转移酶1A1基因多态性和饮食暴露与结直肠癌易感性的关系

目的 研究磺基转移酶（SULT）1A1基因Arg213His多态性与结直肠癌易感性,及其与结直肠癌相关危险因素的交互作用。方法 对140例结直肠癌患者和343例正常对照进行病例对照研究,运用PCR—RFLP方法检测SULT1A1基因Arg213His多态。结果 SULT1A1的Arg／Arg、Arg／His和His／His的分布频率在对照组和结肠癌、直肠癌组中差异无统计学意义（P〉0．05）。红肉、过度烹饪肉类的摄入与结肠癌和直肠癌的发生,在控制年龄、性别、吸烟和既往病史后,关联无统计学意义。红肉摄入量〉5kg／年组结合SULT1A1突变基因型在对照组和直肠癌组差异有统计学意义,OR值为3．78（95％CI：1．08～13．20）。结论 磺基转移酶1A1基因Arg213His多态性与肉类摄入联合在直肠癌发生过程中起着一定作用。

A case-control study on the association between the genetic polymorphism of sulfotransferase 1A1, diet and susceptibility of colorectal cancer

Objective To investigate the relationship between sulfotransferase 1A1 polymorphism, diet and colorectal cancer susceptibility. Methods A case-control study of 140 cancers and 343 health controls was conducted to investigate the role of sulfotransferase 1A1 polymorphism and meat consumption in colorectal carcinogenesis. Genotypes of sulfotransferase 1A1 polymorphism were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results There was no significant difference in allele frequency of SULT1A1 between the control and cancer patient populations. After adjustment for age, sex, smoking and history of diseases, red meat and well-done meat intake showed no significant association with colorectal cancer. Consumption of red meat more than 5 kg per year combined with SULT1A1 slow sulfation (Arg/His and His/His) had a statistically significant association with the risk of rectal cancer (OR = 3.78; 95% CI; 1.08-13. 20) compared to that consumed red meat less than 5 kg per year with fast sulfation (Arg/Arg). Conclusion This study suggests that SULT1A1 slow sulfation combined with higher intake of red meat may be associated with an elevated risk of rectal cancer.