Over-expression and siRNA of a novel environmental lipopolysaccharide-responding gene on the cell cycle of the human hepatoma-derived cell line HepG2 |
| |
| Authors: | Du Kejun Chai Yubo Hou Lichao Chang Wenhui Chen Suming Luo Wenjing Cai Tongjian Zhang Xiaonan Chen Nanchun Chen Yaoming Chen Jingyuan |
| |
| Affiliation: | Department of Occupational and Environmental Health, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, PR China. |
| |
| Abstract: | Lipopolysaccharide (LPS) is the toxic determinant for Gram-negative bacterium infection. The individual response to LPS was related to its gene background. It is necessary to identify new molecules and signaling transduction pathways about LPS. The present study was undertaken to evaluate the effects of a novel environmental lipopolysaccharide-responding (Elrg) gene on the regulation of proliferation and cell cycle of the hepatoma-derived cell line, HepG2. By means of RT-PCR, the new molecule of Elrg was generated from a human dental pulp cell cDNA library. Expression level of Elrg in HepG2 cells was remarkably upgraded by the irritation of LPS. Localization of Elrg in HepG2 cells was positioned mainly in cytoplasm. HepG2 cells were markedly arrested in the G1 phase by over-expressing Elrg. The percentage of HepG2 cells in G1 phase partly decreased after Elrg-siRNA. In conclusion, Elrg is probably correlative with LPS responding. Elrg is probably a new protein in cytoplasm which plays an important role in regulating cell cycle. The results will deepen our understanding about the potential effects of Elrg on the human hepatoma-derived cell line HepG2. |
| |
| Keywords: | FBS, fetal bovine serum FCM, flow cytometry GNB, Gram-negative bacterium HDP, human dental pulp Elrg, Environmental lipopolysaccharide-responding gene LBP, LPS binding protein LPS, lipopolysaccharide LSCM, Laser scanning confocal microscopy MAPK, mitogen-activated protein kinase NF-κB, nuclear factor-κB PRRs, pattern recognition receptors RNAi, RNA interference SABC–FITC, streptavidin–biotin peroxidase complex–fluorescein isothiocyanate shRNA, small hairpin RNA siRNA, small interfering RNA TLR4, toll-like receptor 4 |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
