| 松萝烟酰胺对胃癌SGC-7901细胞增殖、凋亡、细胞周期及迁移的影响 |
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| 引用本文： | 尤雨桐,贺小琼,陆地,连文静,屈倩倩,段灵,周樟屏. 松萝烟酰胺对胃癌SGC-7901细胞增殖、凋亡、细胞周期及迁移的影响[J]. 中草药, 2020, 51(4): 1024-1030 |
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| 作者姓名： | 尤雨桐 贺小琼 陆地 连文静 屈倩倩 段灵 周樟屏 |
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| 作者单位： | 昆明医科大学公共卫生学院, 云南 昆明 650500,昆明医科大学公共卫生学院, 云南 昆明 650500,云南昆明医科大学 生物医学工程中心, 云南 昆明 650500,昆明医科大学公共卫生学院, 云南 昆明 650500,昆明医科大学附属口腔医院, 云南 昆明 650500,昆明医科大学公共卫生学院, 云南 昆明 650500,昆明医科大学公共卫生学院, 云南 昆明 650500 |
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| 基金项目： | 国家自然科学基金资助项目（81760538）；云南省科技厅科技计划项目（2018FE001）；昆明医科大学重大成果培育项目（CGPY201603） |
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| 摘 要： | 目的探讨松萝烟酰胺对胃癌SGC-7901细胞的增殖抑制作用及机制。方法体外培养SGC-7901细胞,分为对照组和不同浓度的松萝烟酰胺实验组,显微镜观察各组细胞形态;MTT法测定SGC-7901细胞增殖;AnnexinV/PI双染和DAPI荧光染色检测细胞凋亡;流式细胞术检测细胞周期;细胞划痕实验检测细胞的迁移。结果松萝烟酰胺处理SGC-7901细胞后,细胞发生皱缩、变形,贴壁细胞脱落;MTT结果显示,松萝烟酰胺对SGC-7901增殖抑制作用呈明显的浓度依赖性和时间依赖性;AnnexinV/PI双染结果显示,松萝烟酰胺使SGC-7901细胞的晚期凋亡率增加,且DAPI染色可观察到明显的细胞凋亡的核浓缩、核碎裂;流式结果显示,松萝烟酰胺使SGC-7901细胞周期停滞在S期;划痕实验显示,随着时间的延长、浓度的增加,松萝烟酰胺使SGC-7901细胞迁移率下降越明显。结论松萝烟酰胺对SGC-7901细胞具有增殖抑制作用,其作用机制可能是通过诱导细胞凋亡、阻滞细胞于S期,从而抑制细胞的迁移。
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| 关 键 词： | 松萝烟酰胺 胃癌SGC-7901细胞 增殖 细胞凋亡 细胞周期 细胞划痕实验 |
| 收稿时间： | 2019-11-06 |
Effects of usnicoyinamide on proliferation, apoptosis, cell cycle, and migration of SGC-7901 gastric cancer cells |
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| YOU Yu-tong,HE Xiao-qiong,LU Di,LIAN Wen-jing,QU Qian-qian,DUAN Ling and ZHOU Zhang-ping. Effects of usnicoyinamide on proliferation, apoptosis, cell cycle, and migration of SGC-7901 gastric cancer cells[J]. Chinese Traditional and Herbal Drugs, 2020, 51(4): 1024-1030 |
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| Authors: | YOU Yu-tong HE Xiao-qiong LU Di LIAN Wen-jing QU Qian-qian DUAN Ling ZHOU Zhang-ping |
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| Affiliation: | School of Public Health, Kunming Medical University, Kunming 650500, China,School of Public Health, Kunming Medical University, Kunming 650500, China,Biomedical Engineering Center of Kunming Medical University, Kunming 650500, China,School of Public Health, Kunming Medical University, Kunming 650500, China,School of Stomatology, Kunming Medical University, Kunming 650500, China,School of Public Health, Kunming Medical University, Kunming 650500, China and School of Public Health, Kunming Medical University, Kunming 650500, China |
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| Abstract: | Objective To investigate the inhibitory effect of usnicoyinamide on the proliferation of gastric cancer cell line SGC-7901 and its mechanism. Methods SGC-7901 cells were cultured in vitro and divided into two groups:control group and experimental group with different concentrations of usnicoyinamide. The morphology of each group of cells was observed by a microscope; Proliferation of SGC-7901 cells was measured by MTT assay; The mechanism of apoptosis was studied by AnnexinV/PI double staining and DAPI fluorescence staining; Flow cytometry was used to detect the effect of usnicoyinamide on the cell cycle; Effect of usnicoyinamide on invasion and migration of SGC-7901 cells was detected by cell scratch test. Results After SGC-7901 cells were treated with usnicoyinamide, the cells were wrinkled, deformed and adherent cells fell off; The results of MTT showed that the inhibition of the proliferation of SGC-7901 cells was a significant dose-effect relationship and time-dependent; The results of AnnexinV/PI double staining showed that nicotine increased the late apoptosis rate of SGC-7901 cells, and DAPI staining showed obvious nuclear concentration and nuclear fragmentation of apoptosis. The results of flow cytometry showed that the cell cycle of SGC-7901 cells stagnated in S phase; Scratch test showed that the mobility of SGC-7901 cells was decreased more obviously with the prolongation of time and the increase of concentration. Conclusion Usnicoyinamide can inhibit the proliferation of gastric cancer cell line SGC-7901, and its mechanism may be achieved by inducing late apoptosis, inducing S phase cell arrest and inhibiting the invasion and migration of SGC-7901 cells. |
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| Keywords: | usnicoyinamide SGC-7901 gastric cancer cells proliferation apoptosis cell cycle cell wound scratch assay |
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