建立小鼠腹部心脏移植模型的体会

目的:探讨小鼠腹部异位心脏移植手术的技术方法及其改进，为进行移植免疫学研究提供动物模型。方法:采用供心主动脉与受体腹主动脉、供心肺动脉与受体下腔静脉端侧吻合方法对100例小鼠进行腹部心脏移植术，同时测量相关移植部位的重要解剖结构。结果:供心主动脉直径为（1.0±0.1）mm，供心肺动脉直径为（1.3±0.2）mm;受体下腔静脉直径为（1.9±0.2）mm，腹主动脉直径为（0.9±0.1）mm，腹腔大血管平均可利用长度为（6.0±1.0）mm。建模成功率为92%。供、受体手术时间分别为（8.0±1.0）min和（40.2±3.0）min;其中动脉吻合为（9.3±1.2）min，静脉吻合为（7.8±1.7）min。结论:所建立的小鼠心脏移植模型稳定可靠，适用于移植免疫学方面的研究。

Ventral heterotopic heart transplantation in mice

Abstract:Objective:To explore an improved technique of mouse ventral heterotopic heart transplantation and construct an animal model for the study of transplantation immunity.Methods:The donor heart aorta and the recipient ventral aorta, the donor pulmonary artery and the recipient inferior caval vein, were anastomosed by using the end-to-side suture technique respectively. At the same time, important anatomical structures of transplantation-related sites were measured.Results:The diameter of recipient inferior caval vein and ventral aorta was (1.9±0.2)mm and (0.9±0.1)mm respectively. The average length of ventral blood vessels which can be used was (6.0±1.0)mm. The model construction success rate was 92%. The average time of donor operation and recipient operation was (8.0±1.0)min and (40.2±3.0)min respectively. The time of artery anastomosis and vena cava anastomosis was (9.3±1.2)min and (7.8±1.7)min respectively. Conclusions:The animal model is stable and can be used for the study of transplantation immunity.