2010年深圳市手足口病的病原学检测分析

目的:了解深圳市2010年手足口病的病原学情况,为手足口病的控制和治疗提供实验室依据。方法:采集手足口病病例粪便标本,提取标本中的病毒核酸,采用RT-PCR方法,检测肠道病毒71型(EV71)和柯萨奇病毒A16型(CA16)的核酸。结果:2010年共检测1238例手足口病例,其中重症病例122例。所有病例中,EV71阳性率为43.29%,CA16阳性率为11.14%;重症病例中,EV71阳性率75.4%,CA16阳性率仅1.63%;死亡病例均为EV71阳性。5月份病例数最多;城乡结合部为重症病例高发区。3岁以下儿童为手足口病重症高发年龄段。结论:EV71和CA16是引起深圳市手足口病的主要病原体,其中EV71是引起重症病例的最主要原因,必须加强对EV71引起的手足口病病例的监测和治疗。

Pathogenic detection of hand-foot-mouth disease in Shenzhen in 2010

Objective:To understand the pathogenic epidemics of hand-foot-mouth disease(HFMD),and provide laboratory data for the epidemic control and patient care.Methods:Stool samples were collected from HFMD cases to extract RNA,and EV71 and CA16 were detected by RT-PCR method.Results:1238 HFMD samples were detected in 2010,including 122 severe cases.43.29% of 1238 cases were infected by EV71,while 11.14% were infected by CA16.75.4% of 122 severe cases,including 7 death cases,were infected by EV71,while 1.63 were infected by CA16.Most severe cases occurred in rural-urban fringe zone in Shenzhen.Children under 3 years old were at high risk of HFMD severe case.Conclusion:HMFD in Shenzhen were mainly caused by EV71 and CA16,while severe cases were mainly caused by EV71.The monitoring and treatment of HFMD caused by EV71 should be strengthened.