甲氧基聚乙二醇修饰几种红细胞稀有血型抗原的初步研究

目的：研究用甲氧基聚乙二醇(mmthoxy polyethylene glycol，mPEG)修饰红细胞稀有血型抗原，为制备通用型血寻找可能的途径。方法：用3种具有不同瑞基的mPEG修饰红细胞，观察其对红细胞表面4种稀有血型抗原的修饰效果及对红细胞结构、功能和存活期的影响。结果：实验表明，在1．0mmol／L时mPEG-BTC(苯并三唑碳酸酯端基PEG)就可以使红细胞表面Jk^a，Jk^b，k和P1抗原性消失，而对红细胞形态、渗透脆性、自身溶血、乙酰胆碱酯酶、胆固醇、ATP、2，3-DPG含量、变形性以及存活期无影响，其他2种mPEG对红细胞修饰效果不好。结论：mPEG-BTC(可有效遮蔽红细胞表面的稀有血型抗原，对其结构、功能及存活期无影响，本实验为mPEG包裹法制备通用型血提供了有力的实验证据。

Preliminary study on camouflage of rare blood group antigen on red blood cells with methoxy polyethylene glycol

Objective: To study camouflage of rare blood group antigen on red blood cells with methoxy polyethylene glycol(mPEG), and to find a possible way to prepare universal donor red blood cells( RBCs). Methods: Three kinds of mPEG derivatives were used to modify human RBCs.The camouflage effects of mPEG on four kinds of rare blood group antigen located on RBC surface and the influence of mPEG modification on cell structure and function were observed. Results: The antigenic activity of Jk a, Jk b, k and P 1 on RBC surface disappeared after modification by mPEG-BTC at the concentration of 1.0 mmol/L. There were no harmful effects on cell morphology,structure and function including osmotic fragility,hemolysis,AchE,cholesterol, ATP, 2,3-DPG and deformability. The other two kinds of mPEG could not achieve such camouflage effect. Conclusion: The mPEG-BTC can camouflage the rare blood group antigen of Jk a, Jk b, k and P 1 on RBC surface efficiently and has no influence on structure and function of modified RBCs.