高密度脂蛋白胆固醇试剂对天门冬氨酸氨基转移酶测定结果的影响

目的探讨某国产试剂(B公司)高密度脂蛋白胆固醇(HDL-C)试剂对天门冬氨酸氨基转移酶(A ST)测定结果的影响。方法按照EP 9-A 2文件,实现B公司A ST测定结果与可溯源罗氏公司(A公司)A ST测定结果的可比性。分别单独用A公司、B公司的A ST试剂测定筛选出的40份不同浓度的A ST新鲜血清标本。用上述40份新鲜标本,先用A公司的HDL-C试剂,先进行HDL-C测定,等加完试剂2后,启动紧急停机程序,过反应时间,再用A公司的A ST试剂测定A ST,以保证两个项目前后在同一个比色杯反应。再按同样方法用A公司的HDL-C试剂与B公司的A ST试剂,B公司的HDL-C试剂与B公司的A ST试剂、A公司的A ST试剂测定A ST。结果A公司与B公司的A ST试剂测定结果具有可比性。A公司的HDL-C试剂对A公司和B公司的A ST试剂测定A ST结果与单独用A公司A ST试剂测定A ST结果均具有可比性。B公司的HDL-C试剂对A公司试剂、B公司A ST试剂测定A ST结果与单独用A公司A ST试剂测定A ST的结果之间完全没有可比性。结论由于比色杯的交叉污染,B公司的HDL-C试剂对A公司、B公司A ST试剂测定A ST结果均有严重干扰。

Influence of High Density Lipoprotein Cholesterol Reagent to Interfere Testing Serum Aspartate Aminotransferase

Objective To explore the influence of a homemade high density lipoprotein cholesterol(HDL-C) reagent to interfere testing serum aspartate aminotransferase(AST).Methods It was ensured comparable between the AST results of non-traceable B test system and that of traceable Roche A test system according to EP-9A2 file.AST was deterimined in 40 specimen of different concentration fresh serum by AST reagent of A corporation or B corporation,respectively.HDL-C was determined by HDL-C reagent of A corporation firstly.It was to perform stat interruption procedure when second reagent was added.AST was determined by AST reagent of A corporation after reaction time in order to ensure reaction at same colorimetric cylinder.It was same the method between HDL-C reagent of A corporation and AST reagent of B corporation and AST reagent of A corporation or B corporation.Results There was comparable between the AST results of B test system and that of A test system separately,the results of AST between HDL-C reagent of A corporation and AST reagent of A corporation or B corporation,but it was not comparable the results of AST between HDL-C reagent of B corporation and AST reagent of A corporation or B corporation.Conclusion HDL-C reagent of B corporation was interfered the determination of AST by AST reagent of A corporation and B corporation because of cross-pollution of colorimetric cylinder.