BDNF and TrkB expression in intrastriatal ventral mesencephalic grafts in a rat model of Parkinson's disease |
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| Authors: | J Sautter M Sabel C Sommer S Strecker N Weidner W H Oertel M Kiessling |
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| Institution: | (1) Institute of Physiology, University of Munich,,;(2) Institute of Neuropathology, University of Heidelberg, and,;(3) Department of Neurology, Zentrum für Nervenheilkunde, University of Marburg, Federal Republic of Germany, DE |
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| Abstract: | Summary. We investigated the expression of BDNF and its high affinity receptor trkB in fetal dopaminergic grafts in a rat model of
Parkinson's disease. Grafts were allowed to differentiate for 7, 14, 28, or 56 days, respectively and were analyzed immunocytochemically
thereafter with antibodies directed against tyrosine hydroxylase, BDNF and trkB. At all time points investigated, grafts contained
tyrosine hydroxylase immunoreactive neurons. Immature grafts (7 days) displayed no immunoreactivity for BDNF which was restricted
to glial cells at the graft-host interface. After longer differentiation periods BDNF-immunoreactivity was detectable in neurons
and astrocytes within the grafts. No trkB immunoreactivity was found in immature grafts but a strong signal for trkB emerged
in grafted neurons older than 14 days whereas glial cells remained unlabeled at all time points investigated. Expression of
BDNF and trkB in grafted neurons and of BDNF in sourrounding glial cells suggests an autocrine or paracrine action of BDNF
on dopaminergic neurons possibly mediated by activated glia.
Accepted December 15, 1997; received October 28, 1997 |
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| Keywords: | : Immunocytochemistry dopaminergic neuron neurotrophin transplantation |
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