靶向高分子造影剂的制备及其体外寻靶实验

目的制备一种特异性靶向肝癌细胞的高分子超声造影剂,并考察其体外寻靶能力。方法采用双乳化法制备高分子材料PLGA-COOH造影剂;并用碳二亚胺法将造影剂与抗体偶联制备出靶向高分子造影剂。检测该造影剂一般特性及体外寻靶能力,并与普通高分子造影剂做比较。结果高分子造影剂的平均粒径600.5 nm,体外寻靶试验显示,该靶向高分子造影剂较多并牢固的聚集到肝癌细胞表面;普通高分子造影剂对照组末见造影剂和细胞的结合。结论成功制备出特异性结合人肝癌抗体的靶向高分子超声造影剂。该造影剂在体外对肝癌细胞具有较强的特异性亲合力。

Preparation of PLGA-COOH-targeted Ultrasound Contrast Agent and Targeting Study in Vitro

Objective To prepare a kind of HCC targeted high molecular polymer ultrasound contrast agent and investigate its affinity for HCC in vitro.Methods The high molecular polymer PLGA-COOH ultrasound contrast agents were produced by the double emulsion technique.Targeted high molecular polymer ultrasound contrast agents were prepared by combining PLGA-COOH ultrasound contrast agents with HAB18 monoclonal antibody using EDC/NHS.Its characteristic and the ability of targeting to HCC were determined with non-targeted high molecular polymer ultrasound contrast agents as the control group.Results The average diameter of targeted high molecular polymer ultrasound contrast agents was 600.5 nm,In the targeting study in vitro,it showad that many targeted high molecular polymer ultrasound contrast agents conjugated with HCC tightly whereas none conjugation in the control group.Conclusions HCC targeted high molecular polymer ultrasound contrast agents were prepared successfully.The targeted ultrasound contrast agents can bind to HCC effectively in vitro.