Synthesis of [3α‐3H] 17α‐Hydroxy pregnenolone and [3α‐3H] Pregnenolone |
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| Authors: | Yuan Tian Yang Hong Samuel J Bonacorsi Aaron Balog Sharon Gong |
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| Institution: | 1. Radiochemical Synthesis Group, Bristol–Myers Squibb Company, Princeton, NJ, USA;2. Discovery Chemistry, Bristol–Myers Squibb Company, Princeton, NJ, USA |
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| Abstract: | For the first time, 3α‐3H] 17α‐hydroxy pregnenolone (1) was synthesized through a multiple step sequence. The presence of 3β‐3H] isomer in RP‐HPLC purified product was identified by tritium NMR. The 3β‐3H] isomer was then separated from 3α‐3H] 17α‐hydroxy pregnenolone with chiralPAK AD‐H column. 3α‐3H] pregnenolone (2) was synthesized from commercial available 5‐pregnen‐3,20‐dione in one step with an improved procedure. |
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| Keywords: | tritium 17α ‐hydroxy pregnenolone pregnenolone isomer tritium‐NMR (T‐NMR) chiralPAK AD‐H column |
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