| 吗啡诱发条件性位置偏爱激活时大鼠伏隔核前额叶皮层p-CREB、c-Fos的变化 |
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| 引用本文： | 赵永娜,李晓红,方正梅,魏辉明,邵晓霞.吗啡诱发条件性位置偏爱激活时大鼠伏隔核前额叶皮层p-CREB、c-Fos的变化[J].中华行为医学与脑科学杂志,2008,17(5). |
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| 作者姓名： | 赵永娜 李晓红 方正梅 魏辉明 邵晓霞 |
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| 作者单位： | 1. 昆明医学院药学院,昆明,650031
2. 成都军区昆明总医院麻醉科 |
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| 基金项目： | 云南省教育厅科学技术基金，云南省应用基础研究项目 |
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| 摘 要： | 目的 探讨吗啡诱发条件性位置偏爱激活时大鼠伏隔核、前额叶皮层磷酸化cAMP反应元件结合蛋白(phospho-cAMP response element binding protein,p-CREB)和c-Fos表达的变化.方法 以剂量递增法连续皮下注射(SC)吗啡6d建立大鼠条件性位置偏爱(conditioned place preference,CPP)模型,第7天用生理盐水替代吗啡训练大鼠10d,使形成的cPP逐渐消退,单次SC 4mg·kg-1吗啡激发已消退的CPP.采用免疫组化技术测定吗啡激发CPP重现时大鼠伏隔核、前额叶皮层p-CREB和c-Fos的变化.结果 (1)吗啡可使大鼠产生CPP效应,吗啡4mg·kg-1可使已消失的CPP效应激活;(2)与对照组相比吗啡诱发的CPP激活时大鼠伏隔核、前额叶皮层p-CREB(161.18±5.37,179.75±7.68)和c-Fos(176.63±8.42,185.11±5.61)的表达增加,与NS组比较差异有显著性(P＜0.05).结论 伏隔核、前额叶皮层p-CREB和c-Fos蛋白表达参与了吗啡的CPP重现.
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| 关 键 词： | 吗啡 条件性位置偏爱 伏隔核 前额叶皮层 cAMP反应元件结合蛋白 |
Effect of morphine-reactivated conditioned place preference on P-CREB. c-Fos expression in rat nucleus accumbens and prefrontal cortex |
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| Abstract: | Objective To detect the effect of morphine-reactived conditioned place preference(CPP)on phospho-cAMP response element binding protein(p-CREB)and c-Fos expression in rat nucleus accumbens and prefrontal codex. Methods Morphine was administered by subcutaneous injection at gradually increasing dose tered saline instead of morphine for 10 days to induce CPP extinction. The rats were given a single priming injection immunohistochemistry method in the phase of reoccurrence of CPP. Results(1)Increasing dose of morphine days drug free period. (2)Compared to saline control, morphine-reactivated CPP elevated the expression of PCREB and c-Fos in nucleus accumbens and prefrontal codex in rats(P＜0. 05). Conclusion p-CREB and c-Fos expression in nucleus accumbens, prefrontal cortex may be involved in the mechanisms of reoccurrence of CPP. |
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| Keywords: | c-Fos |
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