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Developing equine mtDNA profiling for forensic application |
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| Authors: | Susan M.R. Gurney Sandra Schneider René Pflugradt Elizabeth Barrett Anna Catharina Forster Bernd Brinkmann Thomas Jansen Peter Forster |
| Affiliation: | 1. Institute of Forensic Genetics, 48161, Münster, Germany 2. Institute of Continuing Education, University of Cambridge, Madingley Hall, Cambridge, CB23 8AQ, UK 3. Department of Genetics, University of Cambridge, Cambridge, CB2 3EH, UK 4. Certagen GmbH, Marie-Curie-Str 1, 53359, Rheinbach, Germany 5. Institute of Legal Medicine, University Hospital Freiburg, Albertstra?e 9, 79104, Freiburg, Germany 6. Kenton, Suffolk, IP14 6JS, UK 7. Russee, 24111, Kiel, Germany 10. Genetic Ancestor Ltd., PO Box?503, Cambridge, CB1 0AN, UK 8. Murray Edwards College, University of Cambridge, Cambridge, CB3 0DF, UK 9. Cambridge Society for the Application of Research, Churchill College, Cambridge, CB3 0DS, UK |
| Abstract: | Horse mtDNA profiling can be useful in forensic work investigating degraded samples, hair shafts or highly dilute samples. Degraded DNA often does not allow sequencing of fragments longer than 200 nucleotides. In this study we therefore search for the most discriminatory sections within the hypervariable horse mtDNA control region. Among a random sample of 39 horses, 32 different sequences were identified in a stretch of 921 nucleotides. The sequences were assigned to the published mtDNA types A–G, and to a newly labelled minor type H. The random match probability within the analysed samples is 3.61%, and the average pairwise sequence difference is 15 nucleotides. In a “sliding window” analysis of 200-nucleotide sections of the mtDNA control region, we find that the known repetitive central motif divides the mtDNA control region into a highly diverse segment and a markedly less discriminatory segment. |
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