施万细胞移植对电针损伤大鼠中脑新生髓鞘的影响

背景髓鞘的再生预示着损伤神经组织的修复状态. 目的观察施万细胞移植于电针损伤的中脑后是否有新生的髓鞘再生. 设计完全随机设计. 地点和对象实验地点为北京市神经外科研究所;研究对象为 Wistar大鼠,雌雄不限,体质量( 180± 20) g.随机分为单纯电针损伤组和施万细胞移植组,每组 18只动物. 干预新生大鼠坐骨神经施万细胞体外培养, 5溴-脱氧尿嘧啶( BrdU)标记后移植至电针损伤的大鼠中脑网状结构.免疫组织化学染色定位后行半薄切片天青-美蓝髓鞘染色,油镜下观察新生的髓鞘. 主要观察指标①移植的施万细胞在脑内的存活时间.②单纯电针损伤组和施万细胞移植组新生的髓鞘. 结果实验过程中单纯电针损伤组死亡 5只,施万细胞移植组死亡 6只,最终进入分析保持为每组 18只.施万细胞移植后 8个月仍可见 BrdU阳性细胞并主要向大脑皮质迁移;在损伤的脑干区域内 1个月就可见新生的髓鞘. 结论施万细胞移植可促进损伤的中枢神经系统再生.

Impact of Schwann cell transplantation on neogenetic myelin sheath of the midbrain in rats injured by electro-acupuncture

BACKGROUND:The neogenesis of myelin sheath predicts the rehabilitative status of the injured nerves. OBJECTIVE:To observe whether there is neogenesis of myelin sheath after Schwann Cell(SC) transplantation in the midbrain injured by electroacupuncture. DESIGN:A completely randomised design. SETTINGS and MATERIALS:Study was conducted in Beijing Neurosurgical Institute. Wistar rats in either gender with a body mass of(180± 20) g were randomly allocated into electrical acupuncture injury group(control group) and SC transplantation group(SC group) with 18 rats each. INTERVENTION:SC obtained from the sciatic nerve of newborn rats was cultured in vitro and transplanted into the reticular structure in the midbrain of rats injured by electro-acupuncture after being marked by 5'Bromodexyuridine(BrdU).Specimens were stained by azure-methylene blue myelin sheath staining after immunohistochemical staining localization followed by semithin section.Neogenetic myelin sheath was observed under oil microscope.MAIN OUTCOME MEASURES:①The survival period of SC transplanted in the brain.② Neogenetic myelin sheath in both control group and SC group. RESULTS:Five rats in control group and 6 rats in SC group died during the study.Eighteen rats of each group were maintained into final analysis.BrdU-positive cell could be seen even at 8 months after SC transplantation, which mainly migrated towards cerebral cortex.Neogenetic myelin sheath could be seen in the injured brain stem at one month. CONCLUSION:SC transplantation can promote the regeneration of the injured central nervous system.