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| 可卡因对性成熟期大鼠睾丸谷胱甘肽过氧化物酶基因表达的影响 | |
| 引用本文： | 贾晓俤,宋天保,史小莲.可卡因对性成熟期大鼠睾丸谷胱甘肽过氧化物酶基因表达的影响[J].中国药物滥用防治杂志,2009,15(1):18-21. |
| 作者姓名： | 贾晓俤 宋天保 史小莲 |
| 作者单位： | 1. 西安交通大学医学院法医系,西安,710061 2. 西安交通大学医学院生殖医学中心,西安,710061 3. 西安交通大学医学院药理系,西安,710061 |
| 摘 要： | 目的：研究可卡因对大鼠睾丸谷胱甘肽过氧化物酶（glutathione peroxidase GSH-PX）基因表达的影响。方法：性成熟期健康SD雄性大鼠皮下注射可卡因制造吸毒动物模型。化学比色法检测睾丸氧化应激指标；半定量RT-PCR方法检测睾丸组织的GSH-PXmRNA表达。结果：可卡因处理第7天，实验组GSH-PX活性、MDA含量与对照组比较无显著性差异（P〉0．05）；而GSH-PXmRNA水平显著高于对照组（P〈0．05）。可卡因处理第14天，实验组GSH-PX活性显著低于对照组（P〈0．05），MDA含量显著高于对照组（P〈0．05），而GSH-PXmRNA水平显著高于对照组（P〈0．05）。可卡因处理第21天，实验组GSH-PX活性及mRNA表达水平显著低于对照组（P〈0．05），MDA含量显著高于对照组（P〈0．05）。可卡因处理第28天，实验组GSH-PX活性及mRNA表达水平显著低于对照组（P〈0．05），MDA含量显著高于对照组（P〈0．05）。 |
| 关 键 词： | 可卡因 基因表达 谷胱甘肽过氧化物酶 大鼠 |
The effect of cocaine on the gene expression of GSH-PX in testis of sexual maturity rats |
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| JIA Xiao-di,SONG Tian-bao,SHI Xiao-lian.The effect of cocaine on the gene expression of GSH-PX in testis of sexual maturity rats[J].Chinese Magazine of Drug Abuse Prevention and Treatment,2009,15(1):18-21. | |
| Authors: | JIA Xiao-di SONG Tian-bao SHI Xiao-lian |
| Institution: | JIA Xiao-di, SONG Tian-bao, SHI Xiao-lian (1 .Department of forensic medicine, Medical School, Xi＇an Jiaotong University, Xi＇an 710061, Shaanxi Province, China; 2. Research Center of Reproductive Medicine, Medical School, Xi＇an Jiaotong University, Xi＇an 710061, Shaanxi Province,China; 3. Department of pharmacology, Medical School, Xi＇an Jiaotong University, Xi＇an 710061, Shaanxi Province,China) |
| Abstract: | Objective： To study the effects of cocaine on the gene expression of glutathione peroxidase （GSH-PX） in testis of sexual maturity rats. Methods： Forty sexual maturity rats were randomly divided into 2 groups.One group served as control. The other group as cocaine exposure group was given cocaine hydrochloride （15 mg/kg body weight ） subcutaneously daily, cocaine exposure group were harvested at 1,2,3 and 4 weeks after treatment. GSH-PX mRNA expression was investigated by semi-quantitative RT-PCR. The level of GSH-PX and MDA in testis was evaluated by using spectrophotometric determination. Results： At first week after treatment,as compared with the control group,the mRNA expression level of GSH-PX in cocaine exposure group increased significantly （P〈0.05）,while the levels of GSH-PX and MDA mildly increased,but no statistically significant difference was observed.At second week after treatment, as compared with the control group,the mRNA expression level of GSH-PX and the level of MDA in cocaine exposure group increased significantly（P〈0.05）, while the level of GSH-PX decreased significantly（P〈0.05）.At third week after treatment,as compared with the control group, the mRNA expression level of GSH-PX and the level of GSH-PX in cocaine exposure group decreased significantly（P〈0.05）;while the level of MDA increased significantly（P〈0.05）. At forth week after treatment,as compared with the control group,the mRNA expression level of GSH-PX and the level of GSH-PX in cocaine exposure group decreased significantly（P〈0.05）;while the level of MDA increased significantly（P〈0.05）. |
| Keywords: | Cocaine Glutathione peroxidase Gene expression Rat |
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