| 核酸扩增技术（NAT）在献血者血液初筛中的应用现状 |
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| 引用本文： | 蔡丽娜,;陈宝安.核酸扩增技术（NAT）在献血者血液初筛中的应用现状[J].中国实验血液学杂志,2014(4):1171-1177. |
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| 作者姓名： | 蔡丽娜 ;陈宝安 |
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| 作者单位： | [1]东南大学医学院附属中大医院血液科; [2]江苏省血液中心检验科,江苏南京210042 |
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| 基金项目： | 江苏省医学重点学科资助项目（2012-12） |
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| 摘 要： | 随着输血在临床中的应用越来越广泛,对输血传播疾病问题的研究也就越来越受到重视.直到20世纪90年代,针对输血传播疾病的初筛完全依赖于血清学试验,除了乙型肝炎病毒（HBV）可以使用乙型肝炎表面抗原（HBsAg）检测以外,其他病毒的检测基本上都是检测抗体的方法.分子技术（如PCR）的出现,为提高献血者的血液初筛检出率,降低经血传播疾病这种风险提供了可能.本文对国内外核酸扩增技术在献血者血液初筛中的应用现状进行了回顾.
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| 关 键 词： | 核酸检测 血液筛查 无偿献血 |
Progress of Improving Blood Donor Screening by Nucleic Acid Technology——Review |
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| Institution: | CAI Li-Na , CHEN Bao-An(1. Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University,Nanjing, 210009, Jiangsu Province, China; 2 Department of Clinical Laboratory, Jiangsu Province Blood Center, Nanjing 210042, Jiangsu Province, China) |
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| Abstract: | With increasing application of blood transfusion, the research of side-effects such as transfusiontransmitted infections（TTls） became more and more important. Up to the 90＇s of the 20th century, the first blood donor screening for pathogens transfected from blood transfusion entirely depended on serological test. At this time, the detection of virus were perphormed mainly by using method of detecting antibody, except hepatitis B virus （HBV） can be detected by hepatitis B surface antigen （HBsAg）. Now, the molecular technologies, such as the polymerase chain reaction（ PCR）, have been used in clinic. These technologic methods can provide capability of detection for blood donor screening and reduc possibility of infection from blood transfusion. This review summarises the development of nucleic acid amplification technology and describes its current state. |
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| Keywords: | NAT blood screening blood donation |
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