丹参酮ⅡA对异丙肾上腺素致心肌缺血损伤大鼠apelin及其受体的影响

目的:观察异丙肾上腺素(ISO)致心肌缺血损伤大鼠的心肌组织中apelin及其受体APJ的变化,探讨丹参酮ⅡA改善其心肌缺血损伤的作用机制.方法:雄性SD大鼠分为正常对照组、ISO组、不同剂量丹参酮ⅡA治疗组.皮下注射ISO建立大鼠心肌缺血损伤模型,ELISA分析检测血浆和心肌apelin和NO的含量,real-time PCR方法检测心肌中apelin及其受体APJmRNA表达,Western blot方法检测心肌组织中APJ、eNOS及其磷酸化蛋白水平.结果:与正常对照组比较,ISO组大鼠血浆和心肌组织apelin和NO含量均下降,心肌组织中apelin和APJ mRNA水平表达下调(P＜0.01),APJ、eNOS磷酸化蛋白水平降低;与ISO组相比,中、高浓度的丹参酮ⅡA可以明显增加大鼠血浆和心肌组织中apelin和NO含量,增加心肌组织中apelin和APJ mRNA表达水平,增加心肌组织中APJ和eNOS磷酸化蛋白水平.结论:丹参酮ⅡA抗大鼠心肌缺血损伤的机制可能与其上调心肌组织apelin/APJ mRNA的表达、提高apelin/APJ蛋白水平、增加eNOS磷酸化水平和NO生成有关.

Effect of tanshinone IIA on changes of apelin and APJ of rats with myocardial ischemia injury induced by isoproterenol

Objective： To investigate the changes of apelin and APJ in myocardium of rats,and discuss the protective mechanism of tanshinone IIA on myocardial ischemia injury induced by isoproterenol.Methods： Male SD rats were divided into normal control group,ISO group,and tanshinone IIA treatment groups with different doses.The model of myocardial ischemia injury was induced by subcutaneous injection of high dose of isoproterenol.ELISA was used to measure the contents of apelin and NO in blood and myocardium.Real-time PCR was used to measure the apelin andAPJ mRNAlevels in myocardium.Western blot was used to detect the APJ and phosphor-eNOS protein levels.Results： Compared with the normal control group,the apelin and NO contents in blood and myocardium were significantly decreased in ISO group,and the mRNA level of apelin/APJ,the protein levels of APJ and ser1177-eNOS in myocardium,were significantly decreased.Compared with ISO group,the apelin and NO contents in blood and myocardium,the mRNA level of apelin/APJ,the protein levels of APJ and ser1177-eNOS in myocardium,were obviously elevated in the media and high dosages of tanshinone IIA groups（P 0.01 or P 0.05）.Conclusion： The results suggested that the protective mechanisms of tanshinone IIAon myocardial ischemia injury may be related to the elevation of apelin/APJ mRNAand protein levels,and the increase of eNOS phosphorylation degree and NO content.