| 高效液相色谱法测定姜黄颗粒中姜黄素成分含量 |
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| 引用本文： | 周颖,刘扬,成旭东.高效液相色谱法测定姜黄颗粒中姜黄素成分含量[J].中国药物警戒,2012,9(4):205-207. |
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| 作者姓名： | 周颖 刘扬 成旭东 |
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| 作者单位： | 1. 苏州市第四人民医院药剂科,江苏苏州,215001
2. 苏州大学药学院,江苏苏州,215123 |
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| 摘 要： | 目的 建立姜黄素高效液相测定方法,测定姜黄颗粒中姜黄素成分含量；方法 采用RP-HPLC,色谱柱Shimpack VP-ODS (4.6mm*150mm,5μm)；流动相:乙腈-4％冰醋酸(48:52)；检测波长:430hm；流速:1mL· min-1；柱温30℃.结果 姜黄素的进样浓度在2.104～105.2μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9998)；平均加样回收率为99.68％,RSD=1.34％(n=9)；结论 该法稳定、简便,可用于姜黄颗粒的质量控制.
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| 关 键 词： | 姜黄颗粒 姜黄素 高效液相色谱法 含量测定 |
Determination of Curcumin in Jianghuang Granules by HPLC |
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| ZHOU Ying
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LIU Yang
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CHENG Xu-dong.Determination of Curcumin in Jianghuang Granules by HPLC[J].Chinese JOurnal of Pharmacovigilance,2012,9(4):205-207. |
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| Authors: | ZHOU Ying LIU Yang CHENG Xu-dong |
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| Institution: | 1Suzhou No.4 People＇s Hospital, Jiangsu Suzhou 215001, China; 2CoUege of Pharmaceutical Science, Soochow University, Jiangsu Suzhou 215123, China) |
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| Abstract: | Objcetive To establish the method for the content determination of curcumin in Jianghuang Granules. Methods HPLC method was adopted. Chromatographic conditions were as follows： Shimpack VP-ODS（150 mm×4.6 mm, 5μm） column was used with mobile phase consisted of acetonitrile-4% phosphoric acid（48：52） at a flow rate of 1.0 mL＇min-1 under a detection wavelength at 430nm. The column temperature was 30℃. Results The linear range of Curcumin was 2.104-105.2μg·mL-1 （r=0.999 8） with an average recovery rate of 99.68%（RSD=1.34%, n=9）. Conclusion The method is simple, reliable and accurate, and can be used for the quality control of Jianghuang Granules. |
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| Keywords: | Jianghuang Granules curcumin HPLC content determination |
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