| Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats |
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| 引用本文： | DU Chengyou,XUE Dong,YANG Chuang,LI Dewei. Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats[J]. 内分泌外科杂志, 2007, 1(1): 17-21 |
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| 作者姓名： | DU Chengyou XUE Dong YANG Chuang LI Dewei |
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| 作者单位： | Department of Hepatobiliary Surgery ＆ Liver Transplantation Center, The First Affiliated Hospital of Chongqing Medical University Chongqing 400016 |
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| 摘 要： | Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.
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| 关 键 词： | CK-19 PDX-1 Nestin Ngn3 供体 纯度 免疫组织化学 干细胞 RT-PCR |
| 文章编号： | 1673-9558（2007）01-0017-05 |
Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats |
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| DU Chengyou,XUE Dong,YANG Chuang,LI Dewei. Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats[J]. , 2007, 1(1): 17-21 |
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| Authors: | DU Chengyou XUE Dong YANG Chuang LI Dewei |
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| Abstract: | Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet. |
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| Keywords: | Donor islet Stem cells Immunohistochemistry RT-PCR Analysis Nestin rats purity islet donor stem cells difference significant purification Results amplified immunohistochemistry mRNA levels protein Islet sediment Group isolated |
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