学龄双生子儿童焦虑抑郁与5-HTTLPR基因多态性的关系

目的 了解5-HTTLPR 基因多态性与学龄双生子儿童焦虑抑郁的关系。方法 选取内蒙古呼和浩特市和包头市8~12 岁147 对双生子儿童(同卵双生子47 对;异卵双生子100 对)为调查对象,运用Achenbach 儿童行为量表(CBCL)测算儿童焦虑抑郁因子得分;采集所有儿童口腔上皮细胞并提取DNA,运用聚合酶链式反应(PCR)方法对5-HTTLPR 基因进行分型;利用广义估计方程(GEE)分析5-HTTLPR 基因多态性及家庭环境因素对学龄双生子儿童焦虑抑郁的影响。结果 LS 和SS 基因型儿童的焦虑抑郁因子得分高于LL 基因型儿童(χ²=3.938,P<0.05);5-HTTLPR 基因型与家庭亲密度(χ²=6.129,P<0.05)和家庭教养方式(χ²=7.665,P<0.05)的交互作用对儿童焦虑抑郁因子得分影响显著。结论 5-HTTLPR 基因型与学龄双生子儿童焦虑抑郁因子得分显著相关;在高亲密度和专制型教养方式的家庭环境中,S 等位基因可能增加双生子儿童焦虑抑郁的可能性。

Association between anxiety-depression and 5-HTTLPR gene polymorphism in school-aged twins

Objective To investigate the association between anxiety-depression and 5-HTTLPR gene polymorphism in school-aged twins. Methods A total of 147 pairs of twins (47 pairs of monozygotic twins, 100 pairs of dizygotic twins) aged 8-12 years from Baotou and Hohhot were selected as respondents. The Achenbach Child Behavior Checklist (CBCL) was used to calculate the scores of anxiety-depression factors in school-aged twins. The DNA was extracted from oral epithelial cells, and polymerase chain reaction was applied for 5-HTTLPR genotyping. The generalized estimating equation (GEE) was used to analyze the effect of 5-HTTLPR polymorphism and family environment on anxiety-depression in school-aged twins. Results The children with LS and SS genotypes had significantly higher scores of anxiety-depression factors than those with LL genotype (χ2=3.938, Pχ2=6.129 and 7.665, both PConclusions 5-HTTLPR genotype is significantly correlated with the scores of anxiety-depression factors in school-aged twins. In the family with high cohesion and an autocratic family rearing pattern, S allele may increase the possibility of anxiety-depression in twin children.