紫球藻B—藻红蛋白的分离纯化

紫球藻（Porphyridium cruentum)的藻胆蛋白粗提物经硫酸铵沉淀及透析后，分别用羟基磷灰石（HA）和Sephadex G-100两种方法柱层析，均可分离纯化出B－藻红蛋白（B－PE），纯度（A545nm/A280nm)分别为4．92和3．78，两种方法纯化后的B－PE在聚丙烯酰胺梯度凝胶电泳时均得到一条带，B－PE的最大吸收峰在545nm，其室温荧光发射峰为574．5nm。

Separation and purification of B-Phycoerythrin in Porphyridium cruentum

The B Phycoerythrin(B PE) could both be separated after crude phycobiliproteins from Porphyridium cruentum were precipitated in ammonium sulphate and dialyzed,then chromatographed on a hydroxylapatite (HA) Column and a Sephadex G 100 column respectively.The purity of B PE separated by two methods was 4.92 and 3.78 (A 545 nm/A 280 nm) respectively.When the purified B PE was electrophoresed under polyacryiamide gradient gel electrophoresis condition,only one band was observed.The B PE possessed an absorption maximum at 545nm and a room fluoresence emission at 574.5nm.