外周血淋巴细胞、单核细胞激光散射参数与异型淋巴细胞的相关性

目的 分析淋巴细胞和单核细胞两类白细胞（WBC）的体积、电导率、激光散射（vCS）参数与异型淋巴细胞的相关性.方法 采用Sysmex-800i血液分析仪进行血常规检测的129例病毒感染患者和50例正常体检者的检测结果回顾性分析,统计其淋巴细胞体积（MLV）、分布宽度（LDW）、电导率（MLC）、激光散射（MLS）和单核细胞体积（MMV）、分布宽度（MDW）、电导率（MMC）、激光散射（MMS）.并根据异型淋巴细胞比率将病毒感染患者分为感染1组（异型率2％～5％）、感染2组（异型率＞5％～10％）和感染2组（异型率＞10％）,正常体检者作为对照组,比较四组VCS参数.结果 感染1组LDW为（17.2±2.5）fl,对照组为（14.1±2.7）fl,其差异有统计学意义（t=2.362,P＜0.05）；感染2组的MLV、LDW、MMV、MDW分别为（85.3±5.6）×109个/L、（19.0 ±3.1）fl、（185.9±9.2） ×109个/L、（22.0 ±2.9）fl,均明显高于对照组（t=2.557、3.086、2.735、4.269,均P＜0.05）；感染3组的MLV、LDW、MMV、MDW分别为（89.5±6.8）×109个/L、（22.3 ±3.6）fl、（194.5±9.6） ×109个/L、（24.5 ±3.4）fl,均明显高于对照组（t=2.982、3.569、2.945、4.863,均P＜0.05）；三组感染患者的MLC、MLS、MMC、MMS的分别为感染1组（112.3±7.8） μs/cm、（54.8±4.9） NTU、（112.3±7.8） μs/cm、（83.6±4.7）NTU,感染2组（104.7±6.9） μs/cm、（52.6 ±4.5）NTU、（104.7±6.9）μs/cm、（81.1±4.3）NTU,感染3组（96.5±6.2） μs/cm、（50.5 ±4.3）NTU、（96.5±6.2） μs/cm、（78.7 ±3.8）NTU,三组均明显低于对照组（t=2.623、3.195、2.247、5.829、2.457、2.956、2.371、6.715、2.573、3.044、4.395、7.386,均P＜0.05）.结论 淋巴细胞和单核细胞两类白细胞的VCS参数能够在一定程度上反应外周血异型淋巴细胞的形态学改变,对临床感染疾病诊断和治疗有参考应用价值.

The relationship between VCS parameters of peripheral blood lymphocyte,monocyte and atypical lymphocyte

Objective To analyze the relationship between volume,conductivity,laser light scatter （VCS） of lymphocytes and monocytes and atypical lymphocyte. Methods The clinical data of 129 patients with virus infection and 50 healthy controls received blood routine examination by Sysmex-800i hematology analyzer in our hospital were retrospectively analyzed. The lymphocyte volume （ MLV）, distribution width, （ LDW）, conductance rate （ MLC ）, laser scattering （MLS） and monocyte cell volume （ MMV）, distribution width, （ MDW）, electrical conductivity （ M MC ）, laser scattering（MMS） were analyzed. And according to the heterotypic lymphocyte ratio, the virus infection patients were divided into group 1 （ abnormal rate 2% -5% ）, group 2 （ abnormal rate 〉 5% -10% ）, group 3 （ abnormal rate 〉 10% ）. The healthy controls were selected as control group. The VCS parameters of the four groups were compared. Results The LDW of group 1 was （ 17.2 ± 2.5） FL, that of the control group was （ 14.1 ± 2.7） FL, the difference was statistically significant（t = 2. 362, P 〈 0.05 ）. The MLV, LDW, MMV, MDW of group 2 were （85.3 ± 5.6） ×10^个/L（19 ± 3.1 ） FL, （ 185.9 ± 9.2） × 10^/L, （22 ± 2.9 ） FL, which were significantly higher than those of the control group （t = 2. 557、3. 086、2. 735、4. 269、 P 〈 O. 05 ）. The MLV, LDW, MMV, MDW of group 3 were （ 89.5 ± 6. 8） × 10^9/L, （22.3 ± 3.6） FL, （ 194.5 ± 9.6） × 10^9/L, （24.5 ± 3.4 ） FL, which were significantly higher than those in the control group （t = 2.982,3. 569,2. 945,4. 863,P 〈 0.05 ）. The MLC, MLS, MMC, MMS of group 1 were （112.3 ±7.8）μs/cm,（54.8 ±4.9） NTU,（ll2.3 ±7.8）μs/cm,（83.6 ±4.7） NTU,those in group 2 were （104. 7 ±6.9） μs/cm, （52.6 ± 4.5 ） NTU, （ 104.7 ± 6.9） μs/cm, （81.1 ± 4.3 ） NTU, those in group 3 were （96.5 ± 6.2） μs/cm, （50.5 ± 4.3 ） NTU, （96.5 ± 6.2）μs/cm, （78.7 ± 3.8 ）NTU, those in the three groups were significantly lower than in the control group （t = 2. 623、3 195、2. 247、5. 829、2. 457、2. 956、2. 371、6. 715、2. 573、3. 044、4. 395、7. 386, P 〈 0.05 ）. Conclusion VCS parameters of lymphocytes and mononuclear cells of two types of white blood cellscan reflect to a certain extent, peripheral blood heterotypic lymphocyte morphology changes of clinical infection, has the reference value of diagnosis and. treatment of disease.