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| 丹参对衰老鼠脑海马神经细胞凋亡作用的研究 | |
| 引用本文： | 朱健,蔡文纬,陈朝婷,成静,张静敏,沈佰华.丹参对衰老鼠脑海马神经细胞凋亡作用的研究[J].中国老年学杂志,2001,21(1):46-48. |
| 作者姓名： | 朱健 蔡文纬 陈朝婷 成静 张静敏 沈佰华 |
| 作者单位： | 1. 上海第二医科大学附属第九人民医院老年医学科, 2. 白求恩医科大学药学院, 3. 上海第二医科大学免疫学研究所, |
| 基金项目： | 上海市老年保健科研基金赞助项目(96410 5) |
| 摘 要： | 目的：研究丹参对衰老鼠脑海马神经细胞凋亡的作用。方法：用TUNEL法及流式细胞仪观察衰老鼠的神经细胞凋亡，并用免疫组织化学法检测相关基因表达变化。以丹参注射液干预衰老的大鼠模型，观察了丹参对衰老鼠神经细胞凋亡的作用。结果：衰老鼠海马细胞有典型的凋亡特征，与丹参组比较海马细胞的凋亡率有明显差异，衰老鼠bcl-2表达下调，bax的表达量明显增加，基因表达变化与流式细胞仪测定的细胞凋亡率一致。结论：丹参对衰老鼠的脑神经细胞凋有明显的抑制作用。 |
| 关 键 词： | 衰老 丹参 海马神经细胞 细胞凋亡 |
| 文章编号： | 1005-9202(2001)01-0046-03 |
| 修稿时间： | 1999年10月25 |
Effect of radix salivae miltiorrhizae on apoptosi s of hippocampal cells in aging rats brain |
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| ZHU Jian,ZHANG Jing min,SHEN Bai hua et al The Ninth People's Hospital,Shang Hai Second Medical University,Shanghai.Effect of radix salivae miltiorrhizae on apoptosi s of hippocampal cells in aging rats brain[J].Chinese Journal of Gerontology,2001,21(1):46-48. | |
| Authors: | ZHU Jian ZHANG Jing min SHEN Bai hua The Ninth People's Hospital Shang Hai Second Medical University Shanghai |
| Institution: | ZHU Jian,ZHANG Jing min,SHEN Bai hua et al The Ninth People's Hospital,Shang Hai Second Medical University,Shanghai 200011 |
| Abstract: | Objective To study the effect of Radix Salivae Miltiorrhizae(SM) on apoptosis of hippocampal cells in senescing rtas brain.Methods To identify the morphological characters and percent of neuronal apoptosis in hippocampus with TUNEL(teminal deoxynuleotidyl transferase mediated nick end labeling) and FACS scan meanwhile determine the expression of apoptosis relative genes by immunohistochemical stainning.After intervening the aging group by SM,the effect of SM on spoptosis of hippocampal cells was observed.Results There was typical apoptotic morphological characters in aging group's neuron cells.The percent of neuronal apoptosis in aging group was much higher than that in SM group.The expression of bcl 2 was decreased and bax was increased in aging group significantly.The percentage of neuronal apoptosis consistented with relative genes expression.Conclusions SM was found to inhibit the apoptosis occurring in aging rats hippocampus obviously. |
| Keywords: | Aging Apoptosis Radix salivease miltiorrhizae |
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