| RP-HPLC法测定野菊花中蒙花苷含量 |
| |
| 引用本文： | 谭晓杰,贾英,陈晓辉,毕开顺. RP-HPLC法测定野菊花中蒙花苷含量[J]. 沈阳药科大学学报, 2004, 21(6): 434-435,464 |
| |
| 作者姓名： | 谭晓杰 贾英 陈晓辉 毕开顺 |
| |
| 作者单位： | 沈阳药科大学,药学院,辽宁,沈阳,110016 |
| |
| 摘 要： | 目的建立野菊花中蒙花苷的含量测定方法。方法采用反相高效液相色谱法 ,DiamonsilTMC18柱 (2 5 0mm× 4 6mm ,5 μm) ,流动相为甲醇 水 磷酸 (体积比 5 7∶4 3∶0 0 4 ) ,检测波长为32 6nm。结果蒙花苷线性范围 0 4 8～ 9 70mg·L-1,r =0 9999(n =7) ;平均回收率为 98 4 % ,RSD为 2 0 % (n =9)。结论该测定方法为野菊花药材的质量控制提供依据
|
| 关 键 词： | 野菊花 蒙花苷 反相高效液相色谱法 |
| 文章编号： | 1006-2858(2004)06-0434-02 |
Determination of content of linarin in Flos Chrysanthemi Indici by RP-HPLC |
| |
| TAN Xiao-jie,JIA Ying,CHEN Xiao-hui,BI Kai-shun. Determination of content of linarin in Flos Chrysanthemi Indici by RP-HPLC[J]. Journal of Shenyang Pharmaceutical University, 2004, 21(6): 434-435,464 |
| |
| Authors: | TAN Xiao-jie JIA Ying CHEN Xiao-hui BI Kai-shun |
| |
| Abstract: | Objective To establish a method for the determination of the content of linarin in Flos Chrysanthemi Indici by RP-HPLC.Methods The chromatographic condition included a DiamonsilTM C_(18) column ((250 mm)×(4.6 mm),(5 μm)) and the mobile phase consisting of methanol-water-phosphoric acid (V∶V∶(V=)57∶43∶0.04) and the absorbance was monitored at (326 nm).Results The calibration curve was linear over the range of 0.48～(9.70 mg·L~(-1)) for linarin (r=(0.999 9)).The average recovery was 98.4%(n=9)and the RSD was 2.0%.Conclusion The method can be used for the quality control of Flos Chrysanthemi Indici. |
| |
| Keywords: | Flos Chrysanthemi Indici linarin RP-HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
