冬虫夏草含药血清诱导神经干细胞定向分化的影响

目的探讨冬虫夏草中含药血清诱导神经干细胞（NSCs）定向分化的作用。方法取新生24 h内的Wistar大鼠大脑海马区分离培养神经干细胞三代后,加入低、中、高剂量冬虫夏草含药血清与对照血清进行培养,观察其对神经干细胞影响,通过MTT检测神经细胞生长曲线和免疫组化法检测神经干细胞向神经元分化的情况。结果新生大鼠海马分离培养的神经克隆球,经免疫细胞化学染色检测为Nestin阳性细胞。神经干细胞贴壁分化的细胞呈NSE、GFAP阳性。冬虫夏草低、中剂量组诱导神经干细胞分化为神经元的阳性细胞明显高于对照组,且呈量效依赖关系,高剂量组与对照组没有明显差别。结论冬虫夏草含药血清可体外诱导神经干细胞向神经元方向分化,在一定范围内存在量效依赖关系。

Directed differentiation of neural stem cell induced by aweto serum

Objective To study the effect of aweto serum on directed differentiation of neural stem cells in hippocampi of newborn rats.Methods Hippocampi of newborn Wistar rats within 24 hours were isolated and three generations of neural stem cells were cultured.Low,medium and high doses of aweto serum were added in culture medium and their effects on neural stem cells were observed and compared with that of control serum.Nerve cell growth curve was detected by MTT and the differentiation of neural stem cells into neurons was examed by immunohistochemistry.Results The isolated hippocampal nerve clone ball was identified as Nestin-positive cells after immunocytochemistry detection.The differentiated cells adhering to NSC were NSE and GFAP positive.More positive neurons differentiated by neural stem cells were observed in low and medium dose of aweto serum group,and the effect was in proportion with the dose.There was no difference between high dose of aweto serum group and control group.Conclusion The aweto containing serum can induce the differentiation of neural stem cell into neurons in vitro and the effect is in dependence with the dose to some extent.