外周血单个核细胞表面HLA-A和HLA-B在结直肠肿瘤中的表达

目的检测外周血单个核细胞表面（PBMC）HLA-A和HLA-B在结直肠肿瘤患者中的表达，并探讨其在肿瘤发生发展中的作用。方法以Beta-actin为内参照，实时荧光定量RT-PCR方法检测结直肠肿瘤患者（结直肠肿瘤组，n=50）、结直肠良性病变患者（良性病变组，n=35）和健康体检者(正常对照组，n=42)外周血单个核细胞HLA-A和HLA-B mRNA的表达，并比较结直肠肿瘤不同TNM分期中HLA-A和HLA-B mRNA的表达。三组平均年龄相近。用Rest( relative expression software tool)软件计算相对表达率（Ratio）。结果正常对照组PBMC的HLA-A和HLA-B mRNA相对表达率分别为0.99±0.47，1.13±0.60；结直肠良性病变组相对表达率分别为0.76±0.59，0.84±0.57;结直肠肿瘤组分别为0.38±0.31和0.39±0.35。结直肠肿瘤组PBMC的HLA-A mRNA和HLA-B mRNA相对表达率低于正常对照组和结直肠良性病变组（P＜0.001）。结直肠肿瘤组HLA-A mRNA在TNM不同分期之间的表达无统计学差异(P＞0.05)；HLA-B mRNA在TNMⅠ期患者中的表达高于Ⅱ期和Ⅳ期（P＜0.05），在其他不同分期之间差异无统计学意义(P＞0.05)。结论结直肠肿瘤患者外周血单个核细胞HLA-A和HLA-B与其细胞免疫相关，在抗肿瘤免疫中起重要作用。

Expressions of HLA-A and B mRNA on peripheral blood mononuclear cells in colorectal cancer patients in tumorigenesis

To investigate the expressions of HLA-A mRNA and HLA-B mRNA in colorectal cancer and their roles in tumorigenesis. MethodsBeta-actin was used as a reference gene to normalize the results. The expression levels of HLA-A and -B mRNA on peripheral blood mononuclear cells (PBMC) were determined by real-time PCR in 50 patients with colorectal cancer, 35 patients with benign colorectal lesions and 42 healthy volunteers whose mean age was similar. The PCR data were analyzed by REST software (relative expression software tool) to get the relative expression ratio of HLA-A and -B mRNA in the three groups. ResultsThe relative expression ratio of PBMC HLA-A and -B mRNA was respectively 0.99±0.47 and 1.13±0.6 in the control group, 0.76±0.59 and 0.84±0.57 in the benign colorectal lesion group and 0.38±0.31 and 0.39±0.35 in the colorectal cancer group. The expression levels of HLA-A and -B mRNA on PBMC in the colorectal cancer group were significantly lower than those in the benign colorectal lesion group and the control group. In the colorectal cancer group, the relative expression ratio of HLA-A mRNA had no significant difference in different expression level in the TNM classificationⅠgroup was significantly higher than that in the TNM classificationⅡ and Ⅳ groups(P＜0.05), and there were no significant differences of HLA-B mRNA among different TNM classifications. ConclusionHLA-A and -B mRNA on PBMC in colorectal cancer are related to host immunity and play important roles in the process of anti-cancer.