| HPLC-ELSD测定田七花叶颗粒中人参皂苷Rb3的含量 |
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| 引用本文： | 袁杰. HPLC-ELSD测定田七花叶颗粒中人参皂苷Rb3的含量[J]. 安徽医药, 2008, 12(5): 410-411 |
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| 作者姓名： | 袁杰 |
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| 作者单位： | 安徽省药品检验所中药室,安徼,合肥,230051 |
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| 摘 要： | 目的建立测定田七花叶颗粒中的人参皂苷Rb3含量的方法。方法HPLC-ELSD采用Shim-pack VP-ODS色谱柱，流动相A为乙腈，B为水，梯度洗脱。蒸发光散射检测器漂移管温度105℃，载气流速1．5L·min^-1。结果人参皂苷Rb3在0.81～20．25μg范围内线性关系良好（r=0．9999），平均回收率为99．5％（RSD=0．4％，n=6）。结论本方法操作简便快捷，灵敏准确，可用于田七花叶颗粒的质量控制。
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| 关 键 词： | 田七花叶颗粒 HPLC-ELSD 人参皂苷Rb3 含量测定 |
Determination of notoginsenoside Rb3 in tianqihuaye granules by HPLC-EISD |
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| YUAN Jie. Determination of notoginsenoside Rb3 in tianqihuaye granules by HPLC-EISD[J]. Anhui Medical and Pharmaceutical Journal, 2008, 12(5): 410-411 |
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| Authors: | YUAN Jie |
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| Affiliation: | YUAN Jie(Anhui Institute for Drug Control, Hefei,Anhui 230051) |
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| Abstract: | Aim To establish a method for determination of notoginsenoside Rb3 in tianqihuaye granules. Method HPLC-ELSD was performed on Shim-pack VP-ODS column with acentonitrile（A） and water（B） gradient elution. The temperature of drift was 105℃ and the nebulizer nitrogen flow rate was 1.5 L·min^-1. Result The linear range of notoginsenoside was 0.81-20.25 μg （r=0.9999）,and the average recovery was 99.5% （RSD=0.4%, n=6）. Conclusion A sensitive, accurate and reliable method was developed for the determination of notoginsenoside in tianqihuaye granules and can be applied to control the quality of this preparation effectively. |
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| Keywords: | tianqihuaye granules HPLC-ELSD determination |
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