大鼠在体肺低温缺血再灌注损伤模型的建立

目的:为探讨缺血-再灌注(IR)对移植肺损伤的可能机制,建立大鼠在体肺低温IR损伤模型。方法:模拟临床肺移植建立大鼠在体肺低温IR损伤模型。分别于游离左肺门(对照组,A组)、缺血4h(缺血组,B组)和缺血4h再灌注4h(IR组,C组)后测定动脉血氧分压(PaO2)、动脉血二氧化碳分压(PaCO2)、气道峰压(Paw)以及支气管肺泡灌洗液(BALF)中蛋白含量和左肺含水量,并取左肺组织作病理学观察。结果:所有动物术后全部存活。PaO2、Paw、BALF蛋白含量及左肺含水量,C组与A组及B组之间的差异显著(P<0.01);三组间PaCO2无明显差异(P>0.05);肺病理改变以C组最严重。结论:该方法的建立为研究IR对移植肺的损害机制提供了较为理想的动物模型。

A HYPOTHERMIC MODEL IN VIVO OF LUNG ISHEMIA-REPERFUSION INJURY IN RATS

Objective:To study the possible mechanisms of ishchemia- reperfusion( IR) injury to the function of transplanted lung, we established a hypothermic model in vivo of lung ischemia - reperfusion injury in rats. Methods; 18 SD rats were divided into 3 groups(6 rats per group), control group (Group A), ischemic injury group (Group B), IR group(Group C). In the experiment, we simulated the clinical course of lung transplantation and left hilus of lung was temporarily clamped for 4h ischemia, namely 30 min warm ischemia,3h hypothermic ischemia and 30 min warm ischemia again. Then the counter- lateral lung was clipped after isolating left hilus of lung (group A) , 4h ischemia (group B) , and 4h reperfusion after 4h ischemia (group C). The changes of partial pressure of oxygen in arterial blood ( PaO2) , partial pressure of carbon dioxide in arterial blood (PaCO2), pressure of air way (Paw), bronchoalveolar lavage fluid (BALF) albumin, water content of left lung and pathological study were recorded. Results:The survival rate was 100% postoperatively. For PaO2, Paw, BALF albumin and water content of left lung, there was a significant difference between group C and group A or group B(P<0.01). For PaCO2, no significant difference between the 3 groups was found (P>0.05).The pathological lesions in group B was milder than that in group C, but more serious than that in group A.Conclusions:The study provides a simple, reliable and inexpensive model for determining the possible mchanisms of I/R injury to the function of transplanted lung.