大鼠三叉神经脊束尾侧亚核内Cdk5/p39的表达与活性

目的:探讨周期依赖性蛋白激酶5(Cdk5)2种激活剂p35和p39在出生后各发育阶段大鼠三叉神经脊束尾侧亚核内的表达变化.方法:采用Western印迹分析、免疫沉降和Cdk5活性分析,检测出生后各发育阶段大鼠三叉神经脊束尾侧亚核内Cdk5的活性变化和Cdk5、p39的表达水平.采用SPSS11.0软件包中的两两比较和扩展t检验进行统计学分析.结果:在新生大鼠三叉神经脊束尾侧亚核组织中,p39表达较低;出生后2～3周时,p39的表达最高;到成体时,其表达降低到与新生大鼠三叉神经脊束尾侧亚核组织中的相同水平.在出生后各发育阶段,大鼠三叉神经脊束尾侧亚核内Cdk5的表达一致,而Cdk5的活性逐渐减弱,新生大鼠三叉神经脊束尾侧亚核内的Cdk5活性(115.5Kepm)比成体中的Cdk5活性(19.0 Kcpm)约高6倍,各组之间均有显著差异(P<0.01).结论:在出生后各发育阶段,大鼠三叉神经脊束尾侧亚核内p39的表达与p35表达结果在时间上有差异,Cdk5/p39和Cdk5/p35在出生后各发育阶段大鼠三叉神经脊束尾侧亚核内可能扮演不同的角色.

Expression and activity of Cdk5/p39 during rats Vc development

PURPOSE: To evaluate the different expression of Cdk5 two activators, p35 and p39 throughout rats Vc development. METHODS: The changes of Cdk5 activity, expression of Cdk5 and p39 in the development in spinal trigeminal subnucleus caudalis(Vc) were studied by Western blotting, immunoprecipitation and kinase assay. Statistical analysis was performed using SPSS11.0 software package. RESULTS: Western blot showed that p39 expression was low at newborn, and highest at the 2-3 week-old rat Vc. In the adult rat Vc, expression declined to the same level as in newborn rat Vc. In contrast, the expression of Cdk5 was constant throughout the development rat Vc. Cdk5 activity in the newborn rat Vc(115.5 Kcpm),which was about 6 times higher than that in normal adult rat Vc(19.0 Kcpm). There was significant difference between each group(P<0.01). CONCLUSIONS: Expression of p35 and p39 is differentially distributed throughout rat Vc. Cdk5/p35 and Cdk5/p39 may play different roles in distinct brain regions during different states of the rat Vc development.