高压氧对脓毒症大鼠膈肌损伤的保护作用

目的 观察高压氧(hyperbaric oxygen,HBO)对大鼠脓毒症时膈肌损伤的保护作用.方法 SPF级成年雄性SD大鼠30只,随机分为假手术组、脓毒症组及HBO治疗组,每组10只.其中假手术组在给予水合氯醛麻醉成功后,从正中线剪开大鼠腹部,然后缝合伤口.脓毒症组和HBO组在麻醉成功后.使用盲肠结扎穿刺(CLP)模型,将大鼠制成脓毒症动物模型,并且HBO组在术后立即行HBO治疗.所有大鼠在术后16 h处死,取左侧膈肌检测肌条收缩力,右侧膈肌检测超氧化物歧化酶(SOD)、丙二醛(MDA)含量,膈肌肌细胞凋亡率及病理切片.结果 脓毒症组与假手术组比较,MDA含量、膈肌肌细胞凋亡率明显上升,SOD含量及肌条收缩力明显下降,2组比较差异有统计学意义(P<0.01).给予HBO治疗后,MDA含量、膈肌细胞凋亡率及肌条收缩力较脓毒症组明显恢复,SOD含量也明显上升,2组比较差异有统计学意义(P<0.01).结论 HBO可有效抑制氧自由基生成,减轻氧化应激反应,对膈肌的结构与功能起保护作用,减轻脓毒症膈肌损伤的程度.

Effects of hyperbaric oxygen on diaphragmatic damage induced by sepsis in rats

Objective To study protective effects of hyperbaric oxygen (HBO) on diaphragmatic damage induced by sepsis in rats. Methods Thirty SD rats were randomly divided into 3 groups: group A (the sham group, n = 10), group B (the sepsis group, n = 10) and group C (the HBO-treatment group, n = 10). Rats of group A were administered abdominal operation, and were then sutured following chloral hydrate anesthesia. For animals in group B and C, the sepsis rat model was developed with cecal ligation and puncture, and rats of group C were immediately treated with HBO. All the animals were sacrificed 16 hours after operation. Then, the left diaphragnmtic muscle was taken to treasure muscular contractility and the fight one to detect concentrations of MDA and SOD. Apoptosis of diaphragnmtic muscle cells and the pathological section examination were also made. Results Concentrations of MDA and rate of diaphragmatic muscle cell apoptosis of group B increased markedly, when compared with those of group A, while SOD concentration and muscular contractility of group B decreased significantly than those of group A, with a marked statistical difference (P < 0.01). Following HBO therapy, concentrations of MDA, SOD, diaphragmatic muscle cell apoptosis rate and muscular contractifity recovered obviously, with obvious statistical differences, when compsrisons were made between the two (P<0.01). Conclusions HBO could effectively inhibit formation of oxygen free radicals, reduce oxidative stress and the level of diaphragmatic injury in sepsis.