牙龈卟啉单胞菌PG0839基因对KB细胞表达白细胞介素1β和Toll样受体4影响的研究

Objective To investigate the effect of PG0839 gene form Porphyromonas gingivalis (Pg) on inflammatory cytokine expression in human oral epidermoid carcinoma KB cell. Methods A mutant in the PG0839 gene of Pg was created by insertional inactivation. Group 1 was chanllenged with PgW83 strain, group 2 with PG0839-defective mutant, and the control group with Dulbecco's modified Eagle's medium only. KB cells were co-cultured with presence of bacteria for 24 h. At the time point of 0. 5, 2, 6, 12 and 24 h, cells were stored in Trizol. The mRNA expression of interleukin-1β (IL-1β) and Toll like recepector-4 (TLR-4) was examined by reverse transcription polymerase chain reaction. Results At2 h and6 h, IL-1β mRNA expression was lower in group 2 than in group 1(2 h: 0.31 ±0. 11 versus 0.95±0.48, P＜0.05; 6 h: 0.57±0.20 versus 1.29±0.55, P＜0.05). At 0. 5 h and6 h, TLR-4mRNA expression was lower in group 2 than in group 1 ( 0. 5 h: 0. 20 ± 0. 09 versus 0. 58 ± 0. 09, P ＜ 0. 05;6 h: 0. 34 ± 0. 04 versus 0. 71 ± 0. 18, P ＜ 0.05 ). Conclusions PG0839 gene may play an important role in Pg-induced inflammatory effects of KB cell.

Effect of PG0839 gene of Porphyromonas gingivalis on inflammatory cytokine in human oral epidermoid carcinoma KB cell