红斑狼疮患者皮损组织中高迁移率族蛋白-1的表达

目的 研究红斑狼疮患者皮损中高迁移率族蛋白-1(HMGB-1)的表达情况,初步探讨其在红斑狼疮发病机制中的作用.方法 采用免疫组织化学及Westem-blot法检测健康人皮肤、盘状红斑狼疮(DLE)患者及系统性红斑狼疮(SLE)患者皮损组织中HMGB-1表达.结果 健康人皮肤组织HMGB-1主要表达于表皮角质形成细胞胞核中.DLE患者皮损区HMGB-1主要表达于真皮浸润的单个核细胞中,表皮角质形成细胞HMGB-1仍为核表达,但阳性表达细胞的百分比较健康人减少(t=11.315.P<0.01);非皮损区HMGB-1表达的部位仍为细胞核表达且阳件细胞百分比与健康人相比差异无统计学意义(P>0.05),同时HMGB-1蛋白表达总量与健康人比较差异无统计学意义(t=0.681,P>0.05);SLE患者皮损中除真皮浸润的单个核细胞仍为阳性表达外,皮损区细胞外及表皮细胞质出现HMGB-1阳性表达,而表皮HMGB-1核表达阳性细胞的百分比低于DLE患者(t=6.821,P<0.01),非皮损区HMGB-1的表达部位及附性细胞百分比与健康人相比差异仍无统计学意义(P>0.05);HMGB-1表达总量分别较健康人及DLE患者显著上调(t=15.494,P<0.01;t=13.221,P<0.01),且其表达强度与SLEDAI及尿蛋白升高相关(r=0.565,P<0.01;OR=1.027,P<0.05).结论 红斑狼疮患者皮损中HMGB-1表达部位及表达水平呈不同程度改变,存在亚细胞移位且表达强度增强,提示HMGB-1可能参与红斑狼疮的炎症机制.

Study on the expression of high mobility group box chromosomal protein 1 in skin lesions in patients with lupus erythematosus

Objective To study the expression of high mobility group box chromosomal protein 1(HMGB-1) in the skin lesions of patients with lupus erythematosus and investigate the role of HMGB-1 in the pathogenesis of lupus erythematosus. Methods Immunohistochemical assay and Western-blot were used to test the expression of HMGB-1 in skin lesions from 20 discoid lupus erythematosus (DLE) patients, 25 systemic lupus erythematosus (SLE) patients and 20 healthy controls. Results In healthy controls, H MGB-1 was mainly expressed in the nucleus of keratinocytes. In skin lesions of DLE patients, HMGB-1 was mainly expressed in mononuclear cells of dermis, but the percentage of positive keratinocytes in epidermis of lesion area was decreased than that of healthy controls (t=11.315, P<0.01). In skins that were not involved,HMGB-1 was also expressed in the nucleus of keratinocytes and there was no difference in the percentage of positive keratinocytes between DLE and healthy controls (P>0.05). By Western-blot, there was no stati-stical significance in total protein between DLE and healthy controls (t=0.681, P>0.05). In SLE, besides the mononuclear cells, HMGB-1 could be detected both in the cytoplasmic and extracellular space in dermis,while the HMGB-1 nuclear expressions in keratinocytes'of epidermis were decreased than those of DLE (t=6.821, P<0.01), and in un-involved skin, HMGB-1 was also expressed in the nucleus of keratinocytes and there was no difference in the percentage of positive keratinocytes with healthy controls (P>0.05). The total protein was increased in SLE than that of healthy controls and DLE patients (t=15.494, P<0.01 ; t=13.221, P<0.01, respectively). The intensity of HMGB-1 was con'elated with SLEDAI and proteinuria (r=0.565, P<0.01,OR=1.027, P<0.05, respectively). Conclusion Compared with healthy controls, there is translocation and alteration of HMGB-1 expression in patients with lupus erythematosus, which indicates that HMGB-1 may be involved in the inflammation of lupus erythematosus.