鬼针草总黄酮部分化学成分研究

目的 对鬼针草中的化学成分进行分离纯化,并筛选出具有抗肝纤维化活性的单体化合物. 方法 采用大孔树脂、硅胶、Sephadex LH-20、MCI小孔树脂、十八烷基硅烷键合硅胶柱等柱层析的方法进行分离纯化,并利用波谱技术进行化合物的鉴定. 应用四甲基偶氮唑蓝( MTT)法体外检测单体化合物对肝星状细胞T6 ( HSC-T6 )的抑制作用. 结果从鬼针草中分离鉴定的化合物为3-羟基乙酰基吲哚(1)、山奈酚(2)、6,7,3′,4′-四羟基橙酮(3)、槲皮素(4)、奥卡宁(5)、松脂素(6)、1,2,13-三羟基-3,11(E)-十三二烯-5,7,9-三炔(7)、异奥卡宁(8)、邻苯二甲酸-双(2′-乙基庚基) 酯(9)、山奈酚-3-O-α-L-鼠李糖苷(10)、(2S/2R)-异奥卡宁-7-O-β-D-(2″,4″,6″-三乙酰基)吡喃葡糖苷(11/12)、2-O-β-D-吡喃葡萄糖苷-1,13-二羟基-3,11 ( E)十三二烯-5,7,9-三炔(13). 结论 化合物6, 9 为首次从鬼针草种属中分离得到. 其中黄酮类化合物2, 4 具有较强的体外抑制HSC-T6细胞的增殖作用.

Chemical constituents from Bidens bipinnata L.

Objective To isolate and identify chemical constituents from Bidens bipinnata L. and screen for the an-ti-fibrosis activity. Methods The extracts were passed through HPD-100 macroporous resin columns with 100%water and 70% ethanol following by macro resin HPD-100, silica gel column chromatography, Sephadex LH-20, MCI and ODS chromatography. The structures of the components were elucidated by NMR methods. The prolifera-tion of HSC-T6 cells was evaluated by MTT. Results 13 components were isolated from the plant including 3-(hydroxyacetyl)indole (1), Kaempferol (2), 6,7,3′,4′-tetrahydroxy-aurone (3), Quercetin (4), Okanin (5), Pinoresinol (6), (3E,11E)-tetradecadiene-5,7,9-tetrayn-1,2,13-triol (7), Isookanin (8), bis (2-ethylheptyl) phythalate (9), kaempferol-3-O-α-L-rhamnoside (10), (2S/2R) isookanin-7-O-β-D-(2″,4″,6″-triacetyl)gluco-pyranoside (11/12), 2-O-β-D-glucosyltri-deca-3,11E-en-5,7,9-tetrayn-1,13-diol (13). Conclusion 13 com-ponents are isolated from Bidens bipinnata L. , among which compounds 6, 9 are isolated from this genus for the first time and compounds 2, 4 could inhibit the proliferation of HSC-T6 cells.