早期脓毒症大鼠心脏变化特点及卡维地洛预先干预作用的实验研究

目的 明确早期脓毒症大鼠心脏变化特点及卡维地洛的预先干预作用. 方法 40 只SD大鼠随机分为脓毒症组( S组)、脓毒症卡维地洛预先干预组( CS组)、卡维地洛对照组(C组)和生理盐水对照组(N组),CS组和C组给予卡维地洛10 mg/( kg·d)灌胃,4周后S组和CS组大鼠尾静脉注射脂多糖(LPS)20 mg/kg制备脓毒症模型,C组和N组注射等容量生理盐水,24 h后检测各组大鼠心率、血压、血清磷酸激酸激酶( CK)、磷酸激酸激酶同工酶( CK-MB)、N末端脑钠肽前体( NT-proBNP )浓度,心肌中超氧化物歧化酶( SOD)、丙二醛( MDA)和三磷酸腺苷( ATP)的含量,观察心肌形态学改变. 结果 卡维地洛可使正常大鼠心率减慢,心肌MDA含量下降、SOD含量升高. LPS可使大鼠心率增快,血清CK、CK-MB、NT-proBNP浓度和心肌MDA含量升高,心肌SOD和ATP含量降低;卡维地洛预先干预可减轻LPS所致大鼠的这些变化. 4 组大鼠血压和心肌形态学变化差异无统计学意义. 结论 早期脓毒症大鼠心率增快,心肌氧化应激,心脏功能受损,心肌能量生成减少. 卡维地洛可增强大鼠抗氧化应激能力,卡维地洛预先干预可改善脓毒症心肌能量代谢和心脏功能,可能与其抗氧化应激作用有关.

Experimental research on the characteristics of early septic rats' hearts and the advanced intervention effects of carvedilol

Objective Trying to demonstrate the characteristics of early septic rats ' hearts changes and the ad-vanced intervention effects of carvedilol. Methods 40 SD rats were randomly divided into 4 groups: sepsis group ( S group ) , carvedilol-advanced-intervention sepsis group ( CS group ) , carvedilol group ( C group ) and control group(N group). The S and CS groups were injected lipopolysaccharide (LPS) (20mg/kg) into caudal vein to in-duce sepsis, the C and N groups were injected with the same volume of normal saline. The carvedilol had been o-rally administered 10 mg/kg once a day for 4 weeks prior to the injection of LPS in C and CS groups. The S and N groups were administered normal saline instead. After 24 hours, the items including heart rate, blood pressure, and the concentrations of serum creatine kinase ( CK ) , creatine kinase isoenzyme-MB ( CK-MB ) , amino-terminal pro-brain natriuretic peptide (NT-proBNP),superoxide dismutase(SOD), malondialdehyde(MDA),andadenosine triphosphate( ATP) in myocardium were detected and compared, and the morphological changes of myocardium were observed. Results Carvedilol could slow down heart rate, decrease myocardial MDA concentration and in-crease myocardial SOD concentration in normal rats. LPS could increase rats' heart rate and concentrations of ser-um CK,CK-MB,NT-proBNP and MDA in myocardium, and it also could decrease concentrations of SOD and ATP in myocardium. Carvedilol-advanced-intervention could reduce the effect of the LPS in the rats. Conclusion Sep-sis in early stage could induce the rises of heart rate, oxidative stress, damages of cardiac function, and reduction of myocardial energy generation of the rats. However, carvedilol can enhance the ability of antioxidant of the rats. Carvedilol-advadanced intervention of sepsis could ameliorate myocardial energy metabolism and cardiac function that may be related to the antioxidant property of carvedilol.