| 用DNAG C含量和DNA同源性测定法鉴定米克戴德军团菌 |
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| 引用本文： | 郭军巧,孙丽华,赵卓,赵汉良.用DNAG C含量和DNA同源性测定法鉴定米克戴德军团菌[J].中国卫生检验杂志,1995(2). |
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| 作者姓名： | 郭军巧 孙丽华 赵卓 赵汉良 |
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| 作者单位： | 辽宁省卫生防疫站，辽宁省卫生防疫站，辽宁省卫生防疫站，辽宁省卫生防疫站 辽宁沈阳 110005，辽宁沈阳 110005，辽宁沈阳 110005，辽宁沈阳 110005 |
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| 摘 要： | 本文采用热变性温度法和液相复性速率法对—轻型特征及血清学反应相似米克戴德军团菌(Lm)的菌株进行了测定,结果表明该菌与标准Lm(C DC株)的DNA G Cmol％相差3.45％,与标准Lm(C DC株)的DNA同源性达81.99％,根据伯杰细菌鉴定手册(1984),可判定该菌株与标准Lm(C DC株)为遗传型一致的类群,即从遗传学角度证明该菌为Lm。
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| 关 键 词： | 米克戴德军团菌 DNA G C含量DNA同源性 |
Identification of Legionella micdadei by Determination of DNA G C mol% and DNA Homology |
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| Guo Junqiao Sun lihua Zhao Zhuo Zhao Hanliang.Identification of Legionella micdadei by Determination of DNA G C mol% and DNA Homology[J].Chinses Journal of Health Laboratory Technology,1995(2). |
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| Authors: | Guo Junqiao Sun lihua Zhao Zhuo Zhao Hanliang |
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| Institution: | Guo Junqiao Sun lihua Zhao Zhuo Zhao Hanliang |
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| Abstract: | A pathogen from the blood of a patient preliminarily identified as Legionella micdadei by methods of phe-notypic characteristics and serology was carried out by Thermal Denaturation Temperature (Tm) and Renatu-ration rate of DNA respectively. The results showed that the difference of DNA G C mol% between the pathogen and standard Lm (CDC strain) was 3. 45% ,
and the DNA homology was 81. 99%. According to the In Bergey's Manual of Systemaic Bacteriology (1984), both the strains should be classified into one genetic group, thus,it can be concluded that the pathogen from the blood of the patient is Legionella micdadei. |
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| Keywords: | Legionella micdadei DNA G C mol % DNA Homology |
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